Light-dependent sequestration of TIMELESS by CRYPTOCHROME
Light-dependent sequestration of TIMELESS by CRYPTOCHROME
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DOI：
10.1126/science.285.5427.553
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发表时间：
1999-07-23
期刊：
影响因子：
56.9
通讯作者：
Kay, SA
中科院分区：
文献类型：
--
作者：
Ceriani, MF;Darlington, TK;Kay, SA
大多数生物体都有生物钟，由负反馈的基因调控回路组成，有助于适应光明和黑暗的循环。在这项研究中，果蝇中参与昼夜光感知的蛋白质CRY以光依赖的方式阻断PERIOD/TIMELESS（PER/TIM）异二聚体复合物的功能。在这些条件下不会发生TIM降解：因此，TIM降解与光对其功能的废除是解偶联的，CRY和TIM是同一复合物的一部分，并以光依赖性方式在酵母中直接相互作用。PER/TIM和CRY影响这些蛋白质复合物的亚细胞分布，这些蛋白质复合物在感知光信号后主要存在于细胞核中。因此，CRY通过直接与生物钟的核心成分相互作用而充当昼夜光感受器。
Most organisms have circadian clocks consisting of negative feedback Loops of gene regulation that facilitate adaptation to cycles of light and darkness. In this study, CRYPTOCHROME (CRY), a protein involved in circadian photoperception in Drosophila, is shown to block the function of PERIOD/TIMELESS (PER/TIM) heterodimeric complexes in a light-dependent fashion. TIM degradation does not occur under these conditions: thus, TIM degradation is uncoupled from abrogation of its function by light, CRY and TIM are part of the same complex and directly interact in yeast in a light-dependent fashion. PER/TIM and CRY influence the subcellular distribution of these protein complexes, which reside primarily in the nucleus after the perception of a light signal. Thus, CRY acts as a circadian photoreceptor by directly interacting with core components of the circadian clock.