Cardioprotective Effects of Tannic Acid on Isoproterenol-Induced Myocardial Injury in Rats: Further Insight into "French Paradox'
Cardioprotective Effects of Tannic Acid on Isoproterenol-Induced Myocardial Injury in Rats: Further Insight into "French Paradox'
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DOI：
10.1002/ptr.5376
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发表时间：
2015-09-01
影响因子：
7.2
通讯作者：
Cui, Wei
中科院分区：
文献类型：
--
作者：
Hu, Xitian;Wang, Hua;Cui, Wei
单宁酸是一种多酚类化合物，具有抗诱变、抗癌、抗菌等多种药理作用。然而，TA的心脏保护作用尚未被报道。为了研究TA对大鼠的保护作用，我们先给大鼠TA 7天，然后用异丙肾上腺素（ISO）灌胃。心肌缺血损伤是通过心电图（ECG）模式、形态学和心脏标记酶的变化来指示的。免疫组化法检测c-fos、c-jun、肿瘤坏死因子- (TNF-)、白细胞介素-1 （IL-1）、裂解caspase-3、-9蛋白表达水平，Western blot法检测凋亡相关蛋白Bax、Bcl-2、caspase-3、核因子κ B （NF-B）活性。TA预处理可以改善形态学和心电图的变化，降低标记酶的活性，抑制凋亡相关蛋白的过度表达，上调抗氧化剂的表达。此外，TA预处理导致Bax/Bcl-2比值降低，TNF-、IL-1、caspase-3、cleaved-caspase-3、-9表达降低。TA具有心脏保护作用，其机制可能与降低Bax/Bcl-2比值、c-fos和c-jun表达、抑制NF-B活化、氧化应激、炎症和细胞凋亡有关。这些发现为“法国悖论”和TA有益作用的潜在机制提供了进一步的见解。版权所有：John Wiley & Sons， Ltd
Tannic acid (TA) is a polyphenolic compound, which has shown diverse pharmacological effects with antimutagenic, anticarcinogenic and antibactericidal properties. However, cardioprotective effects of TA have not been reported. To investigate the protective effects of TA, rats were administered TA for 7days and then intoxicated with isoproterenol (ISO). Myocardial ischemia injury was indicated by changes in electrocardiographic (ECG) patterns, morphology and cardiac marker enzymes. Furthermore, protein expression levels of c-fos, c-jun, tumor necrosis factor- (TNF-), interleukin-1 (IL-1), cleaved-caspase-3 and -9 were analyzed by immunohistochemistry, and activities of apoptosis-related proteins Bax, Bcl-2, caspase-3 and nuclear factor kappa B (NF-B) were detected by Western blot. Pretreatment with TA ameliorated changes in morphology and ECG, reduced activities of marker enzymes, suppressed overexpression of apoptosis-related proteins, upregulated expression of antioxidants. Moreover, TA pretreatment contributed to the decrease in ratio of Bax/Bcl-2, as well as reduced expression of TNF-, IL-1, caspase-3, cleaved-caspase-3 and -9. TA displayed cardioprotective effects, which may be attributed to lowering of Bax/Bcl-2 ratio, c-fos and c-jun expression and inhibition of NF-B activation, as well as oxidative stress, inflammation and apoptosis. These findings provide further insight into the French paradox' and the mechanisms underlying the beneficial effects of TA. Copyright (c) 2015 John Wiley & Sons, Ltd.