Characterization of Axon Guidance Phenotypes in Wnt/PCP Mutant Mice.
Characterization of Axon Guidance Phenotypes in Wnt/PCP Mutant Mice.
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Wnt/PCP 突变小鼠中轴突引导表型的表征。
DOI：
10.1007/978-1-0716-2035-9_17
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Zou,Yimin
中科院分区：
文献类型：
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作者：
Miller,KathrynM;Marfull-Oromí,Pau;Zou,Yimin
我们的实验室发现Wnt家族蛋白可以作为轴突引导分子发挥作用，平面细胞极性(PCP)通路介导了Wnts在轴突引导中的作用。关键证据之一是通过识别基因敲除或条件性基因敲除动物的轴突引导缺陷。我们利用了各种轴突追踪和标记技术，包括免疫组织化学(IHC)、DiI、BDA和荧光记者(GFP或tdTomato)。这些研究主要是在脊髓连合轴突进行的，但也应用于视网膜神经节细胞轴突、皮质脊髓束轴突、多巴胺能和5-羟色胺能投射。
Our lab showed that the Wnt family proteins can function as axon guidance molecules and the planar cell polarity (PCP) pathway mediates the function of Wnts in axon guidance. One of the key evidences was by identifying the axon guidance defects in knockout or conditional knockout animals. We utilized a variety of axon tracing and labeling techniques, including immunohistochemistry (IHC), DiI, BDA, and fluorescent reporters (GFP or tdTomato). These studies have primarily been conducted in spinal cord commissural axons, but have been applied to retinal ganglion cell axons, corticospinal tract axons, dopaminergic and serotonergic projections.