Detection of specific plasma proteins on surfaces by immunospecific adhesion of dyed polystyrene beads.
Detection of specific plasma proteins on surfaces by immunospecific adhesion of dyed polystyrene beads.
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通过染色聚苯乙烯珠的免疫特异性粘附来检测表面上的特定血浆蛋白。
DOI：
10.1163/156856297x00542
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Leonard,EF
中科院分区：
文献类型：
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作者：
Mandrusov,E;Vroman,L;Leonard,EF
本文描述并评估了一种定量生物材料表面吸附的特定血浆蛋白量的方法。特别是，它表明，使用染色聚苯乙烯珠的免疫特异性粘附，可以在至少 12 cm2 的区域内获得评估白蛋白、IgG、纤维蛋白原和 HMK（高分子量激肽原）空间分布的宏观图像（“染色”）。使用扫描仪和图像分析系统测量的染色强度可以量化用于涂覆表面的溶液中特定蛋白质的量。使用所提出的方法获得的结果产生了白蛋白、免疫球蛋白 G (IgG) 和纤维蛋白原的单一蛋白质等温线，其遵循朗缪尔样吸附行为，并且与之前发表的等温线相似。 HMK 等温线也表现出类朗缪尔吸附行为。所提出的方法还检测到纤维蛋白原在玻璃上的吸附是否存在预期最大值，作为血浆稀释的函数。将 6.4% 血浆中的纤维蛋白原吸附到来自分离流的玻璃上，产生的结果表明分离区域边界处纤维蛋白原的数量和位置。这一结果证实了所提出的方法在实际装置中检测从血浆中吸附的特定蛋白质的空间分布的实用性。
This paper describes and evaluates a method for quantifying the amounts of specific plasma proteins adsorbed to biomaterial surfaces. In particular, it demonstrates that macroscopic images (`stains'), that assess the spatial distribution of albumin, IgG, fibrinogen, and HMK (high molecular weight kininogen), can be obtained over areas of at least 12 cm2using immunospecific adhesion of dyed polystyrene beads. Stain intensities, measured with a scanner and an image analysis system, were found to quantify the amount of specific protein in the solution used to coat the surfaces. Results obtained with the proposed method produced single protein isotherms for albumin, immunoglobulin G (IgG) and fibrinogen that followed Langmuir-like adsorption behavior and were similar to previously published isotherms. The HMK isotherm also exhibited Langmuir-like adsorption behavior. The proposed method also detected the presence of an expected maximum in the adsorption of fibrinogen onto glass as a function of plasma dilution. Adsorption of fibrinogen out of 6.4% plasma onto glass from a separated flow produced results indicating the quantity as well as the location of fibrinogen at the boundary of the separated region. This result confirmed the utility of the proposed method for detecting spatial distributions of specific proteins adsorbed from plasma in practical devices.