Identification of the progenitors of Indonesian and Vietnamese avian influenza A (H5N1) viruses from southern China.
Identification of the progenitors of Indonesian and Vietnamese avian influenza A (H5N1) viruses from southern China.
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DOI：
10.1128/jvi.02468-07
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发表时间：
2008-04-01
影响因子：
5.4
通讯作者：
Guan, Y
中科院分区：
文献类型：
--
作者：
Wang, J;Vijaykrishna, D;Guan, Y
高致病性H5N1禽流感病毒在东南亚国家的传播在2003年底引发了第一次重大疫情和传播浪潮，加速了对世界的大流行威胁。由于在这些暴发之前缺乏流感监测，这一时期H5N1病毒的遗传多样性和传播途径仍未确定。为了确定第一波H5N1病毒的可能来源，我们最近对2001年至2004年在中国南部的广东、湖南和云南的活禽市场收集的样本进行了进一步测序和分析。对这一时期的73株H5N1分离株的血凝素和神经氨酸酶基因的系统发育分析显示，中国南方的遗传多样性比以前报道的要大。结果表明，2002年和2003年从云南分离的8种病毒与越南、泰国和马来西亚分离的1枝病毒亚系亲缘关系最密切，2002年和2003年从湖南分离的2种病毒与印度尼西亚分离的2.1枝病毒亚系亲缘关系最密切。对6个内部基因的进一步系统发育分析表明，所有10个病毒都与表面基因保持相似的系统发育关系。10种祖病毒均为Z基因型，在大部分基因片段上与其后代病毒具有较高的相似性（>/=99%）。这些结果表明，2002年和2003年期间，H5N1病毒在云南和越南之间以及湖南和印度尼西亚之间存在直接传播联系。家禽贸易可能是病毒传入越南的原因，而从湖南到印度尼西亚的传播途径尚不清楚。
The transmission of highly pathogenic avian influenza H5N1 virus to Southeast Asian countries triggered the first major outbreak and transmission wave in late 2003, accelerating the pandemic threat to the world. Due to the lack of influenza surveillance prior to these outbreaks, the genetic diversity and the transmission pathways of H5N1 viruses from this period remain undefined. To determine the possible source of the wave 1 H5N1 viruses, we recently conducted further sequencing and analysis of samples collected in live-poultry markets from Guangdong, Hunan, and Yunnan in southern China from 2001 to 2004. Phylogenetic analysis of the hemagglutinin and neuraminidase genes of 73 H5N1 isolates from this period revealed a greater genetic diversity in southern China than previously reported. Moreover, results show that eight viruses isolated from Yunnan in 2002 and 2003 were most closely related to the clade 1 virus sublineage from Vietnam, Thailand, and Malaysia, while two viruses from Hunan in 2002 and 2003 were most closely related to viruses from Indonesia (clade 2.1). Further phylogenetic analyses of the six internal genes showed that all 10 of those viruses maintained similar phylogenetic relationships as the surface genes. The 10 progenitor viruses were genotype Z and shared high similarity (>/=99%) with their corresponding descendant viruses in most gene segments. These results suggest a direct transmission link for H5N1 viruses between Yunnan and Vietnam and also between Hunan and Indonesia during 2002 and 2003. Poultry trade may be responsible for virus introduction to Vietnam, while the transmission route from Hunan to Indonesia remains unclear.