The role of T cells in IgG production; thymus-dependent antigens induce B cell memory in the absence of T cells.
The role of T cells in IgG production; thymus-dependent antigens induce B cell memory in the absence of T cells.
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T 细胞在 IgG 产生中的作用；
DOI：
10.4049/jimmunol.114.6.1665
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发表时间：
1975
影响因子：
4.4
通讯作者：
J. Schrader
中科院分区：
文献类型：
--
作者：
J. Schrader
B细胞记忆显示在注射少量胸腺依赖性抗原，特别是异源血清蛋白，如Fown γ-球蛋白（FGG）或DNP-牛血清白蛋白（DNP-BSA）后，先天无胸腺（nu/nu）小鼠中产生。大剂量的蛋白质（10 mg）倾向于产生特定的B细胞无反应性，尽管仍有一些B细胞引发的证据。抗原不必是多价形式以与B细胞相互作用从而诱导免疫记忆或耐受。与B细胞记忆的诱导相反，发现该系统中IgG抗体的产生强烈依赖于T细胞。胸腺非依赖性抗原如LPS或POL对正常或手术切除胸腺的小鼠的IgG产生具有显著的佐剂作用，不能取代T细胞，从而允许对先天性无胸腺小鼠的胸腺依赖性抗原的IgG应答。然而，T细胞一旦被活化，其作用可能是非抗原特异性的，因为已经显示抗原活化的同源T细胞的上清液刺激了致敏B细胞群培养物中IgG的产生，而不是抗原特异性的。
B cell memory was shown to develop in congenitally athymic (nu/nu) mice after injection with small amounts of thymus-dependent antigens, in particular heterologous serum proteins, such as fown gamma-globulin (FGG) or DNP-bovine-serum albumin (DNP-BSA). Large doses of proteins (10 mg) tended to produce a specific B cell unresponsiveness, although there was still some evidence of B cell priming. The antigen did not have to be in a multivalent form to interact with B cell so as to induce immunologic memory or tolerance. In contrast to the induction of B cell memory, the production of IgG antibody in this system was found to be strongly T cell dependent. Thymus-independent antigens like LPS or POL with pronounced adjuvant effects on IgG production in normal or surgically thymectomized mice, could not replace T cells in allowing an IgG response against thymus-dependent antigens in congenitally athymic mice. However, the action of T cells once activated is likely to be non-antigen-specific, since it was shown that supernatants of antigen-activated-syngeneic T cells stimulated IgG production in cultures of primed B cell populations non-antigen-specifically.