Tissue specific membrane association of alpha 1T, a truncated form of the alpha 1 subunit of the Na pump.
Tissue specific membrane association of alpha 1T, a truncated form of the alpha 1 subunit of the Na pump.
复制标题
α1T 的组织特异性膜关联，α1T 是 Na 泵 α1 亚基的截短形式。
DOI：
10.1016/0014-5793(94)80210-6
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发表时间：
1994
期刊：
影响因子：
3.5
通讯作者：
Medford,RM
中科院分区：
文献类型：
--
作者：
Allen,JC;Pressley,TA;Odebunmi,T;Medford,RM
我们在两种已知含有功能性钠泵位点、VSM微粒体组分（Na+，K+-ATP酶）和完整的原代融合细胞（哇巴因抑制86 Rb摄取）的血管平滑肌（VSM）制备物中，利用定点抗体评估了钠泵α亚基亚型的含量。用肾微粒体比较同种型含量。VSM和肾微粒体均含有全长α1亚基（~100 kDa）以及截短的α 1 T亚基（~66 kDa）。VSM微粒体的SDS处理影响Na+，K+-ATP酶的增加和α 1 T的保留。SDS处理的肾微粒体保留α1亚型和Na+，K+-ATP酶。融合的VSM细胞未检测到α 1 T，仅检测到α 1 T。在缺乏可检测的全长α1的情况下，α 1 T蛋白可能代表犬VSM中的功能性Na泵组分。
We have assessed the Na pump α-subunit isoform content utilizing site directed antibodies in two vascular smooth muscle (VSM) preparations known to contain functional Na pump sites, VSM microsomal fractions (Na+,K+-ATPase) and intact primary confluent cells (ouabain inhibited86Rb uptake). A comparison of isoform content was made with kidney microsomes. Both VSM and kidney microsomes contained a full length α1 subunit (~100 kDa) as well as a truncated subunit, α1T (~66 kDa). SDS treatment of VSM microsomes effected an increase in Na+,K+-ATPase and a retention of α1T. SDS treated kidney microsomes retained the α1 isoform and Na+,K+-ATPase. Confluent VSM cells showed no detectable α1T, only α1T. In the absence of detectable full length α1, the α1T protein may represent a functional Na pump component in canine VSM.