Production and immune response of recombinant Hsp60 and Hsp70 from the salmon pathogen Piscirickettsia salmonis.
Production and immune response of recombinant Hsp60 and Hsp70 from the salmon pathogen Piscirickettsia salmonis.
复制标题
DOI：
10.4067/s0716-97602005000100009
复制
发表时间：
2005
影响因子：
6.7
通讯作者：
V. Wilhelm;C. Soza;Rodrigo Martínez;M. Rosemblatt;L. Burzio;P. Valenzuela
中科院分区：
文献类型：
--
作者：
V. Wilhelm;C. Soza;Rodrigo Martínez;M. Rosemblatt;L. Burzio;P. Valenzuela
我们分离并测序了鲑鱼病原菌pcirickettsia salmonis热休克蛋白60 （Hsp60）和70 （Hsp70）基因。序列分析显示了预期的两个开放阅读框，编码计算分子量为60,060和70,400的蛋白质。这些蛋白与其他已知的原核Hsp60和Hsp70序列具有70-80%的同源性。该编码区已在大肠杆菌中表达为硫氧还蛋白融合蛋白。这两种重组蛋白在大西洋鲑鱼腹腔注射时均能引起体液反应，并对沙门氏菌感染的鱼类具有保护作用。目前的数据将有助于进一步研究热休克蛋白在鱼类对沙门氏菌感染的保护性免疫中的作用，以及它们在针对这种细胞内病原体的重组疫苗中的潜在应用。
We have isolated and sequenced the genes encoding the heat shock proteins 60 (Hsp60) and 70 (Hsp70) of the salmon pathogen Piscirickettsia salmonis. The sequence analysis revealed the expected two open reading frames that encode proteins with calculated molecular weights of 60,060 and 70,400. The proteins exhibit a 70-80% homology with other known prokaryotic Hsp60 and Hsp70 sequences. The coding regions have been expressed in E. coli as thioredoxin fusion proteins. Both recombinant proteins were shown to elicit a humoral response when injected intraperitoneally in Atlantic salmon and also conferred protection to fish challenged with P. salmonis. The present data will facilitate further studies on the involvement of heat shock proteins in protective immunity of fish to infection by P. salmonis and their potential use in recombinants vaccines against this intracellular pathogen.