Functional assessment in the rat by ground reaction forces
Functional assessment in the rat by ground reaction forces
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DOI：
10.1016/s0021-9290(00)00023-3
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发表时间：
2000-06-01
影响因子：
2.4
通讯作者：
Peimer, CA
中科院分区：
文献类型：
--
作者：
Howard, CS;Blakeney, DC;Peimer, CA
尽管大鼠坐骨神经模型被广泛用于修复技术的研究，并且各种评估方法用于评估结果，但缺乏一种直接准确测量这些动物功能恢复的手段。组织学、组织形态计量学和电生理学方法可以作为神经再生的可靠指标，但与功能恢复无关。本研究的目的是研制连续测量地面反作用力（GRF）的装置，并将GRF参数用于评估正常大鼠术前和周围神经断裂修复后的步态参数。对直接坐骨神经修复、直接胫骨神经修复和模拟自体双坐骨神经修复三种神经吻合方法以及胫骨神经横断不修复进行了评价。所设计的测试装置是用客观的数据来测量大鼠的自发和自愿的努力。测量了地面反作用力的三个正交分量——垂直、颅侧（制动和推进）和中外侧。术前数据显示，垂直力在四肢之间具有可比性，但推进力和制动力表现出显著差异。12周时，胫神经直接修复组功能恢复最明显，未修复的胫神经缺损组功能恢复不明显。直接坐骨神经修复和坐骨神经移植的改善程度较小。结果表明，推进力为最优。用于评估有用函数恢复的GRF参数。(C) 2000 Elsevier Science Ltd.版权所有。
Although the rat sciatic nerve model is used extensively in the investigation of repair techniques, and a variety of evaluation methods utilized to assess the results, a means to measure directly and accurately the return of Function in these animals is absent. Histologic, histomorphometric, and electrophysiologic methods can be reliable indicators of nerve regeneration but do not correlate to functional recovery. The purposes of this study were to develop apparatus to continuously measure ground reaction forces (GRF) and use GRF parameters in the assessment of gait parameters in normal rats preoperatively and following peripheral nerve severance and repair. Three neurorrhaphy methods: direct sciatic nerve repair, direct tibial nerve repair and double sciatic nerve repair simulating autograft, as well as a non-repaired tibial nerve transection were evaluated. The testing apparatus was designed to measure the spontaneous and voluntary effort of the rat with objective data. Three orthogonal components - vertical, craniocaudal (braking and propulsion), and mediolateral - of the ground reaction force were measured. Preoperative data showed that vertical forces were comparable among the four limbs but propulsion and braking forces displayed significant differences. At 12 weeks, functional recovery was most evident in the direct tibial nerve repair group and absent in the non-repaired tibial defect group. Direct sciatic nerve repairs and sciatic nerve grafts resulted in lesser degrees of improvement. Results indicated that the propulsive force is the optimal. GRF parameter for evaluating recovery of useful function. (C) 2000 Elsevier Science Ltd. All rights reserved.