Mossy cells in the mouse dentate gyrus: identification in the dorsal hilus and their distribution along the dorsoventral axis
Mossy cells in the mouse dentate gyrus: identification in the dorsal hilus and their distribution along the dorsoventral axis
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DOI：
10.1016/s0006-8993(98)01202-5
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发表时间：
1999-01-23
期刊：
影响因子：
2.9
通讯作者：
Kosaka, T
中科院分区：
文献类型：
--
作者：
Fujise, N;Kosaka, T
以前，我们表明，大多极细胞免疫反应calretinin和亚基2和3的氨基-3-羟基-5-甲基-4-异恶唑丙酸酯（AMPA）型谷氨酸受体（GluR2/3）聚集在腹侧门的小鼠齿状回，并透露，他们是苔藓细胞。虽然这种大的calretinin免疫反应细胞没有看到在背门，我们的高尔基体的研究揭示了苔藓细胞在背门的存在。当我们在背门中观察到大的强烈GluR 2/3免疫反应细胞时，我们表明背门中这些钙视网膜蛋白阴性但GluR 2/3强烈阳性的大细胞也是苔藓细胞。在本研究中，我们用几种方法证实了这种鉴定。细胞外示踪剂标记显示，47个苔藓样细胞均呈GluR2/3强阳性，而钙视网膜蛋白（calretinin）呈阴性，而22个非杂乱的门神经元均无GluR2/3强阳性。电镜下GluR2/3强阳性的胞体和树突具有苔藓样细胞的特征性超微结构。此外，海马伞-穹窿-海马连合横断程序诱导的钙视网膜蛋白的表达在这些背侧GluR2/3免疫反应细胞。根据这些观察结果，我们得出结论，绝大多数强烈GluR2/3免疫反应的大细胞在小鼠背门苔藓。细胞然后，我们评估了假定的差异，苔藓细胞的分布沿着背腹轴的分割器。苔藓细胞的数量密度在腹侧水平比背侧水平大1.4倍，表明苔藓细胞在小鼠门部的分布的背腹差异远小于先前的推测。(C)1999 Elsevier Science B.V.保留所有权利。
Previously we showed that large multipolar cells immunoreactive for calretinin and subunits 2 and 3 of amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) type glutamate receptors (GluR2/3) clustered in the ventral hilus of the mouse dentate gyrus and revealed that they were mossy cells. Although such large calretinin immunoreactive cells were not seen in the dorsal hilus, our Golgi study revealed the presence of mossy cells in the dorsal hilus. As we observed large intensely GluR2/3 immunoreactive cells in the dorsal hilus, we suggested that these calretinin negative but intensely GluR2/3 positive large cells in the dorsal hilus were also mossy cells. In the present study we confirmed this identification with several methods. The extracellular tracer labeling studies revealed that all of 47 mossy cells identified morphologically were intensely GluR2/3 positive but calretinin negative, whereas none of 22 non-messy hilar neurons were intensely GluR2/3 positive. Electron microscopically most of intensely GluR2/3 positive somata and dendritic processes showed the characteristic ultrastructural features of mossy cells. Furthermore, the fimbria-fornix-hippocampal commissure transection procedures induced the calretinin expression in some of these dorsal GluR2/3 immunoreactive cells. On the basis of these observations, we concluded that the vast majority of intensely GluR2/3 immunoreactive large cells in the mouse dorsal hilus were mossy. cells. Then we evaluated the presumed difference in the distribution of mossy cells along the dorsoventral axis by the disector. The numerical density of mossy cells was about 1.4 times larger at the ventral level than at the dorsal level, indicating that the dorsoventral difference in the distribution of mossy cells in the mouse hilus was far smaller than that previously speculated. (C) 1999 Elsevier Science B.V. All rights reserved.