Distinct localization of histone H3 methylation in the vegetative nucleus of lily pollen
Distinct localization of histone H3 methylation in the vegetative nucleus of lily pollen
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DOI：
10.1042/cbi20090124
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发表时间：
2010-03
影响因子：
3.9
通讯作者：
Y. Sano;I. Tanaka
中科院分区：
文献类型：
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作者：
Y. Sano;I. Tanaka
我们分析了百合花粉发育过程中营养细胞核（营养核）中组蛋白H3修饰的分布。在营养核中特异性和/或大量存在的修饰中，在异染色质中发现组蛋白H3在赖氨酸9（H3 K9 me 2）和赖氨酸27（H3 K27 me 2）处的二甲基化，而在营养核中的常染色质中发现组蛋白H3在赖氨酸27（H3 K27 me 3）处的三甲基化。这种独特的定位组蛋白H3甲基化标记，特别是H3 K27 me 3，在细胞核内没有观察到百合细胞核以外的营养核。在花粉成熟过程中，营养核常染色质区H3 K27 me 3的水平增加。结果表明，H3 K27 me 3控制花粉成熟过程中营养细胞的基因表达。
We analysed the distribution of histone H3 modifications in the nucleus of the vegetative cell (the vegetative nucleus) during pollen development in lily (Lilium longiflorum). Among the modifications specifically and/or abundantly present in the vegetative nucleus, dimethylation of histone H3 at lysine 9 (H3K9me2) and lysine 27 (H3K27me2) were found in heterochromatin, whereas trimethylation of histone H3 at lysine 27 (H3K27me3) was localized in euchromatin in the vegetative nucleus. Such unique localization of the histone H3 methylation marks, particularly of H3K27me3, within a nucleus was not observed in lily nuclei other than the vegetative nucleus. The level of H3K27me3 increased in the euchromatic region of the vegetative nucleus during pollen maturation. The results suggest that H3K27me3 controls the gene expression of the vegetative cell during pollen maturation.