Po2 profiles near arterioles and tissue oxygen consumption in rat mesentery
Po2 profiles near arterioles and tissue oxygen consumption in rat mesentery
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DOI：
10.1152/ajpheart.00077.2007
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发表时间：
2007-08-01
影响因子：
4.8
通讯作者：
Pittman, Roland N.
中科院分区：
文献类型：
--
作者：
Golub, Aleksander S.;Barker, Matthew C.;Pittman, Roland N.
大鼠肠系膜中小动脉附近的 PO(2) 分布和组织耗氧量。 Am J Physiol Heart Circ Physiol 293：H1097 - H1106，2007 年。首次发表于 2007 年 5 月 4 日； doi：10.1152/ajpheart.00077.2007。 - 扫描磷光猝灭显微镜技术旨在防止该方法累积的 O(2) 消耗，应用于肠系膜组织中的 PO2 测量。为了进一步提高测量的准确性，将白蛋白结合探针局部应用于组织，并使用物镜安装的加压袋来减少通过肠系膜上薄薄的液体层的氧气运输旁路。在七只麻醉的 Sprague-Dawley 大鼠中，在距离 5、15、30、60、120 和 180 μm 的 84 个肠系膜小动脉（直径 7-39 μm）附近垂直于血液/壁界面的多个部位测量 PO(2)，从而创建 PO2 曲线。发现小动脉上方和紧邻的间质 PO(2) 与已知的血管内值一致。小动脉和大动脉之间的 PO(2) 分布没有发现显着差异，表明毛细血管前肠系膜微脉管系统中存在小的纵向 PO(2) 梯度。此外，PO(2)曲线用于计算肠系膜组织中的耗氧量(56-65nl O(2).cm(-3).s(-1))。针对环境氧气污染对这些值进行校正，得出的耗氧率为 60 - 68 nl O(2).cm(-3).s(-1)，即肠系膜消耗的最大极限。将结果与其他工人就所采用的技术进行的测量进行比较。
PO(2) profiles near arterioles and tissue oxygen consumption in rat mesentery. Am J Physiol Heart Circ Physiol 293: H1097 - H1106, 2007. First published May 4, 2007; doi:10.1152/ajpheart.00077.2007. - A scanning phosphorescence quenching microscopy technique, designed to prevent accumulated O(2) consumption by the method, was applied to PO2 measurements in mesenteric tissue. In an attempt to further increase the accuracy of the measurements, albumin-bound probe was topically applied to the tissue and an objective-mounted pressurized bag was used to reduce the oxygen transport bypass through the thin layer of fluid over the mesentery. PO(2) was measured at multiple sites perpendicular to the blood/wall interface in the vicinity of 84 mesenteric arterioles (7 - 39 mu m in diameter) at distances of 5, 15, 30, 60, 120, and 180 mu m in seven anesthetized Sprague-Dawley rats, thereby creating PO2 profiles. Interstitial PO(2) above and immediately beside arterioles was found to agree with known intravascular values. No significant difference in PO(2) profiles was found between small and large arterioles, indicating a small longitudinal PO(2) gradient in the precapillary mesenteric microvasculature. In addition, the PO(2) profiles were used to calculate oxygen consumption in the mesenteric tissue ( 56 - 65 nl O(2).cm(-3).s(-1)). Correction of these values for contamination with ambient oxygen yielded an oxygen consumption rate of 60 - 68 nl O(2).cm(-3).s(-1), the maximal limit for consumption in the mesentery. The results were compared with measurements made by other workers in regard to the employed techniques.