Somaclonal variation of haploid in vitro tissue culture obtained from Siberian larch (Larix sibirica Ledeb.) megagametophytes for whole genome de novo sequencing
Somaclonal variation of haploid in vitro tissue culture obtained from Siberian larch (Larix sibirica Ledeb.) megagametophytes for whole genome de novo sequencing
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DOI：
10.1007/s11627-014-9619-z
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发表时间：
2014-10-01
影响因子：
2.6
通讯作者：
Vaganov, Eugene A.
中科院分区：
文献类型：
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作者：
Krutovsky, Konstantin V.;Tretyakova, Iraida N.;Vaganov, Eugene A.
本研究的目的是利用巨型配子体外植体从西伯利亚落叶松 (Larix sibirica Ledeb.) 获得遗传稳定的单倍体体外衍生品系，然后将其用于不同的分子遗传学研究，包括全基因组从头测序。然而，11 个微卫星位点的细胞遗传学分析和基因分型显示，在获得的大配子体衍生愈伤组织培养物中，基因组高度不稳定性和突变频率高。所有培养物均在一个或多个微卫星位点中含有新突变。
The objective of this study was to obtain a genetically stable haploid in vitro-derived line from Siberian larch (Larix sibirica Ledeb.) using megagametophyte explants, which then could be used for different molecular genetic studies, including whole genome de novo sequencing. However, cytogenetic analysis and genotyping of 11 microsatellite loci showed high levels of genomic instability and a high frequency of mutation in the obtained megagametophyte-derived callus cultures. All cultures contained new mutations in one or more microsatellite loci.