Development of the microchip-based repeatable immunoassay system for clinical diagnosis
Development of the microchip-based repeatable immunoassay system for clinical diagnosis
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DOI：
10.1088/0957-0233/17/12/s12
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发表时间：
2006-12-01
影响因子：
2.4
通讯作者：
Tokeshi, M.
中科院分区：
文献类型：
--
作者：
Kakuta, M.;Takahashi, H.;Tokeshi, M.
开发了一种基于微芯片的半自动非均相免疫分析方法，其中采用了流动注射样系统和可重复使用的凝胶。微流控技术使用了一个具有高精度流量控制的泵、两个注射器阀和一个微芯片。基于微流体（包括热透镜显微镜作为检测器）染料进样的系统适用性试验结果良好。发现了用于脑钠肽（BNP）的抗原-抗体相互作用的再生缓冲液，其不会失去抗体在凝胶上的结合活性，并且将这样的免疫测定方案整合到半自动微流体系统中将促进重复使用的异质免疫测定。用所选的再生缓冲液进行BNP测定，获得了良好的重复性。使用本文开发的系统和方案成功地测定了0.1-100 pg mL（-1）（n = 3）范围内的BNP浓度。
A microchip-based semi-automated heterogeneous immunoassay was developed wherein a flow injection-like system and repeatable-use gel were employed. Microfluidics used a pump with highly accurate control of flow rate, two injector valves and a microchip. The results of a system suitability test based on dye injection for microfluidics including a thermal lens microscope as a detector were good. A regeneration buffer for antigen-antibody interaction of brain natriuretic peptide (BNP) was found which did not lose binding activity of the antibody on the gels and integration of such an immunoassay scheme into a semi-automated microfluidics system would facilitate repeated-use heterogeneous immunoassay. Good reproducibility of BNP assay was obtained with the selected regeneration buffer. The concentration of BNP in the range of 0.1-100 pg mL(-1) (n = 3) was successfully determined using the system and protocol developed here.