SWITCH PROTEIN ALTERS SPECIFICITY OF RNA-POLYMERASE CONTAINING A COMPARTMENT-SPECIFIC SIGMA FACTOR
SWITCH PROTEIN ALTERS SPECIFICITY OF RNA-POLYMERASE CONTAINING A COMPARTMENT-SPECIFIC SIGMA FACTOR
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DOI：
10.1126/science.2492118
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发表时间：
1989-01-27
期刊：
影响因子：
56.9
通讯作者：
LOSICK, R
中科院分区：
文献类型：
--
作者：
KROOS, L;KUNKEL, B;LOSICK, R
在枯草芽孢杆菌的孢子形成过程中，发育基因spoIVCB和cotD的表达分别在形态阶段IV和V的孢子囊的母细胞室中被诱导。已发现称为σ K（或σ 27）的27千道尔顿RNA聚合酶σ因子，其引起spoIVCB的弱转录和cotD的强转录。还发现了一种14-kD蛋白质，其改变了含σ K的RNA聚合酶的特异性，极大地刺激了spoIVCB转录并显著抑制了cotD转录。σ K和14-kD蛋白都是已知在母细胞中表达特定基因所需的基因的产物。因此，σ K指导母细胞中的基因表达，并且提出14-kD蛋白的失活或隔离在从发育阶段IV到V的过渡期间转换基因表达的时间模式。
During sporulation in Bacillus subtilis, expression of developmental genes spoIVCB and cotD is induced in the mother cell compartment of the sporangium at morphological stages IV and V, respectively. A 27-kilodalton RNA polymerase sigma factor called .sigma.K (or .sigma.27) has been found that causes weak transcription of spoIVCB and strong transcription of cotD. A 14-kD protein was also discovered that changes the specificity of .sigma.K-containing RNA polymerase, greatly stimulating spoIVCB transcription and markedly repressing cotD transcription. Both .sigma.K and the 14-kD protein are products of genes known to be required for expression of specific genes in the mother cell. Thus, .sigma.K directs gene expression in the mother cell and it is proposed that inactivation or sequestering of the 14-kD protein switches the temporal pattern of gene expression during the transition from stages IV to V of development.