Beta2-glycoprotein I (beta2-GPI) mRNA is expressed by several cell types involved in anti-phospholipid syndrome-related tissue damage.
Beta2-glycoprotein I (beta2-GPI) mRNA is expressed by several cell types involved in anti-phospholipid syndrome-related tissue damage.
复制标题
Beta2-糖蛋白 I (beta2-GPI) mRNA 由参与抗磷脂综合征相关组织损伤的几种细胞类型表达。
DOI：
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发表时间：
1999
影响因子：
4.6
通讯作者：
G. Valesini
中科院分区：
文献类型：
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作者：
B. Caronti;C. Calderaro;C. Alessandri;F. Conti;R. Tinghino;G. Palladini;G. Valesini
我们在此报告了参与抗磷脂综合征（APS）病理生理的细胞类型β 2- gpi mRNA的表达，即作为APS自身抗体靶点的内皮细胞、中枢神经系统（CNS）参与APS的星形胶质细胞和神经元。淋巴细胞也被纳入研究，因为已经证明系统性红斑狼疮相关中枢神经系统疾病患者血清中存在与脑抗原交叉反应的抗淋巴细胞抗体，以及鞘内合成的抗神经元抗体。逆转录-聚合酶链反应和限制性内切酶酶切得到的产物表明，在这里测试的所有细胞类型中，无论是否存在胎牛血清，都存在β - gpi mRNA。通过间接免疫荧光技术测定，在两种培养条件下，相同类型的细胞对抗β - gpi MoAb具有免疫反应。综上所述，这些结果表明β - gpi的直接细胞合成，表明β - gpi在APS中具有抗原功能，包括在该综合征中发生的中枢神经系统疾病。
We report here the expression of beta2-GPI mRNA by cell types involved in the pathophysiology of the anti-phospholipid syndrome (APS), i.e. endothelial cells as a target of autoantibodies in the APS, astrocytes and neurones involved in APS of the central nervous system (CNS). Lymphocytes were also included in the study, as it has been demonstrated that patients with systemic lupus erythematosus-associated CNS diseases have serum anti-lymphocyte antibodies cross-reacting with brain antigens, and intrathecally synthesized anti-neurone antibodies. Reverse transcriptase-polymerase chain reaction followed by restriction enzyme digestion of the product obtained demonstrated the presence of beta2-GPI mRNA in all cell types here tested, cultured both in presence and absence of fetal calf serum. In both culture conditions, the same cell types were immunoreactive to an anti-beta2-GPI MoAb, as determined by indirect immunofluorescence technique. Taken together, these results indicate a direct cell synthesis of beta2-GPI, suggesting an antigenic function of beta2-GPI in the APS, including the CNS disease that occurs in this syndrome.