SITES OF INTEGRATION OF INFECTIOUS DNA OF AVIAN RETICULOENDOTHELIOSIS VIRUSES IN DIFFERENT AVIAN CELLULAR DNAS
SITES OF INTEGRATION OF INFECTIOUS DNA OF AVIAN RETICULOENDOTHELIOSIS VIRUSES IN DIFFERENT AVIAN CELLULAR DNAS
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DOI：
10.1016/0092-8674(78)90207-6
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发表时间：
1978-01-01
期刊：
影响因子：
64.5
通讯作者：
TEMIN, HM
中科院分区：
文献类型：
--
作者：
BATTULA, N;TEMIN, HM
确定了两种禽网状内皮组织增殖病病毒感染性DNA的整合模式，这两种病毒的DNA经限制性内切酶Eco RI消化后不会失活。用Eco RI消化来自感染的鸡、火鸡和雉鸡细胞的高分子量DNA，通过琼脂糖凝胶电泳，并检测其感染性。对于用两种网状内皮组织增殖病病毒以高或低感染复数感染的这些禽类细胞，发现了相同的感染性病毒DNA整合模式。在急性感染且伴随细胞死亡的细胞中有多个整合位点。在慢性感染且无细胞死亡的细胞中有单个整合位点。急性感染细胞中每个细胞的整合感染性病毒DNA分子比慢性感染细胞中的多。显然，感染急性期的细胞死亡是感染性病毒DNA在多个位点整合的结果，只有那些感染性病毒DNA仅在单个位点整合的细胞才能存活。
The pattern of integration for the infectious DNA of 2 avian reticuloendotheliosis viruses whose DNA is not inactivated by digestion with the restriction endonuclease, Eco RI, was determined. High MW DNA from infected chicken, turkey and pheasant cells was digested with Eco RI, electrophoresed through agarose gels and assayed for infectivity. The same patterns of integration of infectious viral DNA were found for these species of avian cells infected at high or low multiplicities with 2 reticuloendotheliosis viruses. There were multiple sites of integration in acutely infected cells with concomitant cell death. There was a single site of integration in chronically infected cells with no cell death. There were more integrated infectious viral DNA molecules per cell in acutely infected cells than in chronically infected cells. Apparently, the cell death in the acute phase of infection is a result of the integration of the infectious viral DNA at multiple sites, and only those cells survive that have the infectious viral DNA integrated exclusively at the single site.