Directed differentiation of granular cells from crayfish hematopoietic tissue cells
Directed differentiation of granular cells from crayfish hematopoietic tissue cells
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小龙虾造血组织细胞定向分化为颗粒细胞。
DOI：
10.1016/j.fsi.2019.02.054
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发表时间：
2019-05-01
影响因子：
4.7
通讯作者：
Yang，Feng
中科院分区：
文献类型：
--
作者：
Li，Fang;Xu，Limei;Yang，Feng
血细胞是甲壳类动物的主要免疫细胞。在这些动物的整个生命周期中，都需要产生新的血细胞来维持免疫系统的功能。甲壳类动物的造血机制才刚刚开始了解，需要新的方法来研究这一过程。在这里，我们报道了体外定向分化的颗粒细胞（GCs），从造血组织（HPT）细胞的cherax quadricarinatus。我们首先为培养的HPT细胞提供不同的添加剂来诱导可能的分化。我们发现，小龙虾肌肉提取物对细胞的物理状态有明显的促进作用，并诱导了折光性细胞质颗粒的形成。标记基因的转录和功能性酚氧化酶的产生进一步证实了成熟gc的形成。在我们的实验中，年轻的GCs通常在诱导后2周开始发育，超过60%的细胞在3-4周内成熟。这是首次在体外完成甲壳类动物血细胞的完全分化。为深入研究甲壳类动物的造血功能提供了有力的工具。
Hemocytes are the major immune cells of crustaceans. New hemocyte production is required throughout the life cycle of these animals to maintain a functional immune system. The mechanism of crustacean hematopoiesis has just begun to be understood and new methods are needed for the investigation of this process. Here we report the directed differentiation of granular cells (GCs) from the hematopoietic tissue (HPT) cells ofCherax quadricarinatusin vitro. We started by providing the cultured HPT cells with different additives to induce possible differentiation. We found that crayfish muscle extract greatly promoted the physical status of the cells and induced the formation of refractile cytoplasmic granules. The transcription of marker genes and the production of functional prophenoloxidase further confirmed the formation of mature GCs. In our experiments, young GCs usually started to develop in ∼2 weeks post induction and over 60% of the cells became mature within 3–4 weeks. This is the first time that the fully differentiation of crustacean hemocytes is accomplishedin vitro. It provides a powerful tool for in-depth study of crustacean hematopoiesis.