A model for evaluation of arterial thrombosis following interventional procedures.
A model for evaluation of arterial thrombosis following interventional procedures.
复制标题
评估介入手术后动脉血栓形成的模型。
DOI：
10.1007/pl00011344
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发表时间：
2001
影响因子：
2.1
通讯作者：
Seeger,JM
中科院分区：
文献类型：
--
作者：
Abeia,GS;Hage-Korban,E;Tomaru,T;Barbeau,GR;Friedl,SE;Kaelin,LD;Seeger,JM
． 采用多种介入系统评估血管损伤后血栓形成的模型。该模型由一个“可拉伸的”分流盒组成，作为狗颈动脉和颈静脉之间的动静脉分流。从颈动脉和股动脉获得的动脉同种移植物被安装在连接到分流盒两侧的两个塑料连接器之间。然后将分流箱的相对壁分开，以达到动脉的原始长度。箱体的动脉侧与同侧颈动脉相连，静脉侧与对侧颈静脉相连。止血阀放置在分流箱静脉侧的出口。这些被用作通往同种移植物管腔的各种介入性导管的通道。在开始血流之前进行干预。干预后，在分流箱的动脉侧注射111铟标记血小板，动脉血流开始通过分流器。经分流箱循环1小时后，阻断血流，用戊二醛灌注固定同种移植物，取段进行放射性计数和组织学处理。然后使用该分流箱比较球囊血管成形术（BA）与直接激光血管成形术（LA）和激光热血管成形术（LTA）后血小板粘附和血栓形成。共使用了7只狗的28条动脉。在每个实验中，一个同种移植物作为对照，另外三个同种移植物分别用BA、LA或LTA处理。在干预之后，注射111铟标记的血小板，并使用狗的自然循环循环一小时。BA组标记血小板计数（19102±4869/cm2，平均值±SE）分别显著高于LA组（7038±980/cm2）、热LTA组（5189±1961/cm2）和对照组（1575±541/cm2） （p<0.05，方差分析）。组织学检查显示LA、LTA和对照部位血小板较少，而BA处理部位血小板粘附广泛。该模型提供了在相似条件下对几种干预措施进行同时比较的手段。在这种情况下，热处理的动脉同种移植物有最少的血小板粘附。
. A model to assess thrombus formation following vascular injury was evaluated using various interventional systems. The model consisted of a ‘stretchable’ shunt box that served as an arteriovenous shunt between the carotid artery and jugular vein in dogs. Arterial homografts obtained from both carotid and femoral arteries were mounted between two plastic connectors attached to either side of the shunt box. The opposing walls of the shunt box were then stretched apart to achieve the original length of the arteries. The arterial side of the box was connected to the ipsilateral carotid artery and the venous side was connected to the contralateral jugular vein. Haemostasis valves were placed at the exit ports on the venous side of the shunt box. These were used as an access to the various interventional catheters into the lumens of the homografts. Interventions were performed prior to initiating blood flow. After the interventions,111indium-labelled platelets were injected on the arterial side of the shunt box and arterial blood flow initiated across the shunt. After one hour of circulation through the shunt box, the blood flow was interrupted, and the homografts were perfusion-fixed with glutaraldehyde and segments removed for radioactive counts and processed for histology.This shunt box was then used to compare platelet adhesion and thrombus formation after balloon angioplasty (BA) to direct laser (LA) and laser-thermal angioplasty (LTA). A total of 28 arteries were used from seven dogs. In each experiment, one homograft was used as control, and three other homografts were treated with either BA, LA or LTA. Following the interventions,111indium-labelled platelets were injected and circulated for one hour using the dog's native circulation. Labelled platelet counts for BA (19102±4869/cm2; mean±SE) were significantly greater than LA (7038±980/cm2), thermal LTA (5189±1961/cm2), and control (1575±541/cm2), respectively (p<0.05, ANOVA). Histology examination showed few platelets at LA, LTA and control sites whereas extensive platelet adhesion was noted at BA treated sites. The model provided a means to conduct simultaneous comparison of several interventions under similar conditions. In this case thermal treatment of the arterial homografts had the least amount of platelet adhesion.