Anterior circulation mouse model of subarachnoid hemorrhage
Anterior circulation mouse model of subarachnoid hemorrhage
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DOI：
10.1016/j.brainres.2009.08.021
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发表时间：
2009-10-12
期刊：
影响因子：
2.9
通讯作者：
Macdonald, R. Loch
中科院分区：
文献类型：
--
作者：
Sabri, Mohammed;Jeon, Hyojin;Macdonald, R. Loch
首先在大鼠中描述的蛛网膜下腔出血（SAH）模型，将血液注射到交叉前池中，然后将其应用于小鼠。假设这种前循环 SAH 模型会比其他小鼠模型产生更严重和持续时间更长的血管痉挛。目标是创建一个可用于转基因和基因敲除动物的小鼠模型，以进一步了解蛛网膜下腔出血和血管痉挛。将一根针立体定向地插入视交叉前池，并在几秒钟内注入 100 PI 自体动脉血 (n=10)。将效果与注射盐水（n=10）或假手术（n=7）进行比较。通过激光多普勒监测脑血流量显示，两组注射期间的脑血流量下降具有统计学相似性。 SAH 后 7 天，大脑中动脉和大脑前动脉出现血管痉挛（与注射生理盐水组相比，SAE 中 MCA 半径减少 51%，P
A model of subarachnoid hemorrhage (SAH) first described in rats where blood is injected into the prechiasmatic cistern was adapted to mice. The hypothesis was that such an anterior circulation SAH model would produce vasospasm of greater severity and longer duration than other mouse models. The goal was to create a mouse model that could then be used in transgenic and knockout animals in order to further knowledge of SAH and vasospasm. A needle was inserted stereotactically into the prechiasmatic cistern and 100 PI autologous arterial blood injected over seconds (n=10). Effects were compared to injection of saline (n=10) or to sham operation (n=7). Monitoring of cerebral blood flow by laser Doppler showed a statistically similar decrease during injection in both groups. 7 days after SAH there was vasospasm of the middle and anterior cerebral arteries (51% reduction in MCA radius in SAE compared to saline-injected group, P