Effects of catecholamines on calvarial bone resorption in vitro.
Effects of catecholamines on calvarial bone resorption in vitro.
复制标题
儿茶酚胺对体外颅骨骨吸收的影响。
DOI：
10.1177/000348940111000715
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Chole,RA
中科院分区：
文献类型：
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作者：
Sherman,BE;Chole,RA
许多重要的耳鼻喉科疾病的表现是通过异常的骨重建或破坏。这种病理性重塑的机制仍然知之甚少。已知骨受含去甲肾上腺素的交感神经支配，并且已知交感神经切除术诱导骨吸收。然而，去甲肾上腺素作为一种潜在的骨调节物质的作用尚不清楚。使用颅骨钙释放测定法，我们进行了以下实验以评价去甲肾上腺素、α-激动剂章鱼胺和β-激动剂异丙肾上腺素的骨调节活性。在有和没有甲状旁腺激素的情况下，在2种浓度下测试每种药物。发现去甲肾上腺素对钙释放没有影响。10- 8 mol/l章鱼胺对甲状旁腺素引起的钙释放有明显的刺激作用，10- 6 mol/l异丙肾上腺素对甲状旁腺素引起的钙释放有明显的抑制作用。这项研究表明，一个双峰，浓度依赖性，受体特异性模型的儿茶酚胺介导的调制骨吸收可能在颅骨。
Many important diseases in otolaryngology manifest through abnormal bone remodeling or destruction. The mechanisms for such pathological remodeling remain poorly understood. Bone is known to be innervated by norepinephrine-containing sympathetic nerves, and sympathectomy is known to induce bone resorption. The role, however, of norepinephrine as a potential bone-modulatory substance is unknown. Using the calvarial calcium release assay, we conducted the following experiment to evaluate the bone-modulatory activity of norepinephrine, the alpha-agonist octopamine, and the beta-agonist isoproterenol. Each agent was tested at 2 concentrations with and without parathyroid hormone. Norepinephrine was found to have no effect on calcium release. In contrast, octopamine at 10–8mol/l exerted a significantstimulatoryeffect on calcium release, and isoproterenol at 10–6mol/l exerted a significantinhibitoryeffect on parathyroid hormone-induced calcium release. The investigation suggests that a bimodal, concentration-dependent, receptor-specific model for catecholamine-mediated modulation of bone resorption may operate in calvarial bone.