Comparative transcriptome analysis during early fruit development between three seedy citrus genotypes and their seedless mutants.
Comparative transcriptome analysis during early fruit development between three seedy citrus genotypes and their seedless mutants.
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DOI：
10.1038/hortres.2017.41
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发表时间：
2017
影响因子：
8.7
通讯作者：
Stover E
中科院分区：
文献类型：
--
作者：
Zhang S;Shi Q;Albrecht U;Shatters RG Jr;Stange R;McCollum G;Zhang S;Fan C;Stover E
在无核突变体中鉴定具有差异转录本丰度（GDTA）的基因可能会增强对无核柑橘发育的理解。以Fallglo（Bower citrus hybrid（Citrus reticulata×C.网花C. paradisi）×Temple（C.网花C. sinensis））、葡萄柚（C. paradisi）、菠萝甜橙子（C. sinensis）和它们的无核突变体。在开花后26天（时间点2）观察到无核突变体中的种子败育。Affyphin转录组学分析揭示了359至1077个探针集，在每个柑橘基因型和时间点的无籽与有籽果实的比较中具有差异转录丰度。通过qPCR验证18个微阵列探针组鉴定的GDTA。系统聚类分析揭示了一系列与发育、激素和蛋白质代谢相关的GDTA，这些都可能反映了与无核果实发育相关的基因。分别在时间点1、2和3，在所有三个无核与有籽基因型比较中发现14、9和12个基因表现出相似的丰度比。这些基因中包括编码天冬氨酸蛋白酶和半胱氨酸蛋白酶的基因，它们可能在无核果实发育中起重要作用。对无核柑橘果实发育的新见解可能有助于通过生物技术方法创造无核品种。
Identification of genes with differential transcript abundance (GDTA) in seedless mutants may enhance understanding of seedless citrus development. Transcriptome analysis was conducted at three time points during early fruit development (Phase 1) of three seedy citrus genotypes: Fallglo (Bower citrus hybrid (Citrus reticulata×C. reticulata×C. paradisi)×Temple (C. reticulata×C. sinensis)), grapefruit (C. paradisi), Pineapple sweet orange (C. sinensis), and their seedless mutants. Seed abortion in seedless mutants was observed at 26 days post anthesis (Time point 2). Affymetrix transcriptomic analysis revealed 359 to 1077 probe sets with differential transcript abundance in the comparison of seedless versus seedy fruits for each citrus genotypes and time points. The GDTA identified by 18 microarray probe sets were validated by qPCR. Hierarchical clustering analysis revealed a range of GDTA associated with development, hormone and protein metabolism, all of which may reflect genes associated with seedless fruit development. There were 14, 9 and 12 genes found exhibiting similar abundance ratios in all three seedless versus seedy genotype comparisons at time point 1, 2 and 3, respectively. Among those genes were genes coding for an aspartic protease and a cysteine protease, which may play important roles in seedless fruit development. New insights into seedless citrus fruit development may contribute to biotech approaches to create seedless cultivars.
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