Uncovering novel mobile genetic elements and their dynamics through an extra-chromosomal sequencing approach.
Uncovering novel mobile genetic elements and their dynamics through an extra-chromosomal sequencing approach.
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DOI：
10.1080/2159256x.2016.1189987
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发表时间：
2016-07-01
期刊：
影响因子：
--
通讯作者：
Fischetti, Vincent A
中科院分区：
文献类型：
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作者：
Deutsch, Douglas R;Utter, Bryan;Fischetti, Vincent A
Staphylococcus aureus is a major clinically important pathogen with well-studied phage contributions to its virulence potential. In this commentary, we describe our method to enrich and sequence stealth extra-chromosomal DNA elements in the bacterial cell, allowing the identification of novel extra-chromosomal prophages in S. aureus clinical strains. Extra-chromosomal sequencing is a useful and broadly applicable tool to study bacterial genomics, giving a temporal glance at the extra-chromosomal compartment of the cell and allowing researchers to uncover lower-copy plasmidial elements (e.g., prophages) as well as gain a greater understanding of mobile genetic elements that shuffle on and off the chromosome. Here, we describe how episomal and plasmidial DNA elements can have profound downstream effects on the host cell and surrounding bacterial population, and discuss specific examples of their importance in Gram-positive bacteria. We also offer potential avenues of future research where extra-chromosomal sequencing may play a key role in our understanding of the complete virulence potential of infectious bacteria.
