Purification of exosome-like vesicles from urine.
Purification of exosome-like vesicles from urine.
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DOI：
10.1016/b978-0-12-397945-2.00013-5
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发表时间：
2013
影响因子：
--
通讯作者：
Ward, Christopher J.
中科院分区：
文献类型：
--
作者：
Chen, Christopher Y.;Hogan, Marie C.;Ward, Christopher J.
尿外泌体样囊泡（ELV），由肾上皮脱落的20- 200 nm膜结合颗粒，正在成为监测肾脏疾病的蛋白质、mRNA和miRNA生物标志物的重要来源。然而，ELV的纯化受到大量尿蛋白Tamm-Horsfall蛋白（THP）存在的影响。THP分子低聚成几微米长的双螺旋链。这些线性组装体形成三维凝胶，其在任何基于离心的方案中捕获和隔离ELV。在这里，我们提出了一个纯化协议，分离ELV从THP和尿ELV分为三个不同的群体。
Urinary exosome-like vesicles (ELVs), 20–200nm membrane bound particles shed by renal epithelium, are emerging as an important source of protein, mRNA, and miRNA biomarkers to monitor renal disease. However, purification of ELVs is compromised by the presence of large amounts of the urinary protein Tamm-Horsfall Protein (THP). THP molecules oligomerize into long, double-helical strands several microns long. These linear assemblies form a 3-dimensional gel which traps and sequesters ELVs in any centrifugation based protocol. Here we present a purification protocol that separates ELVs from THP and divides urinary ELVs into three distinct populations.