Both matrix proteins of Ebola virus contribute to the regulation of viral genome replication and transcription
Both matrix proteins of Ebola virus contribute to the regulation of viral genome replication and transcription
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DOI：
10.1016/j.virol.2010.04.002
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发表时间：
2010-07-20
期刊：
影响因子：
3.7
通讯作者：
Becker, S.
中科院分区：
文献类型：
--
作者：
Hoenen, T.;Jung, S.;Becker, S.
埃博拉病毒（EBOV）在人类和非人类灵长类动物中引起严重的出血热。虽然EBOV主要基质蛋白VP 40在形态发生中的作用已被很好地理解，但关于其对病毒基因组复制和/或转录的调节的贡献尚不清楚。类似地，虽然有报道称次要基质蛋白VP 24损害病毒基因组复制，但仍不清楚它是否也调节转录，因为所有常见的实验系统都测量复制和转录的组合产物。我们已经开发出基于qRT-PCR和复制缺陷型微型基因组的系统，可以独立监测病毒的转录和复制。使用这些系统，我们表明，VP 24不仅调节病毒基因组复制，而且转录。此外，我们首次表明VP 40也参与调节这些过程。这些功能在EBOV物种中是保守的，并且在VP 40的情况下，独立于其出芽或RNA结合功能。(C)2010年爱思唯尔公司All rights reserved.
Ebola virus (EBOV) causes severe hemorrhagic fevers in humans and non-human primates. While the role of the EBOV major matrix protein VP40 in morphogenesis is well understood, nothing is known about its contributions to the regulation of viral genome replication and/or transcription. Similarly, while it was reported that the minor matrix protein VP24 impairs viral genome replication, it remains unclear whether it also regulates transcription, since all common experimental systems measure the combined products of replication and transcription. We have developed systems that allow the independent monitoring of viral transcription and replication, based on qRT-PCR and a replication-deficient minigenome. Using these systems we show that VP24 regulates not only viral genome replication, but also transcription. Further, we show for the first time that VP40 is also involved in regulating these processes. These functions are conserved among EBOV species and, in the case of VP40, independent of its budding or RNA-binding functions. (C) 2010 Elsevier Inc. All rights reserved.