Microfluidic organs-on-chips
Microfluidic organs-on-chips
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DOI：
10.1038/nbt.2989
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发表时间：
2014-08-01
影响因子：
46.9
通讯作者：
Ingber, Donald E.
中科院分区：
文献类型：
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作者：
Bhatia, Sangeeta N.;Ingber, Donald E.
芯片上器官是用微芯片制造方法创建的微流体细胞培养装置，其包含由活细胞居住的连续灌注室，所述活细胞被布置成模拟组织和器官水平的生理学。通过重现多细胞结构、组织-组织界面、物理化学微环境和身体的血管灌注，这些装置产生了传统2D或3D培养系统不可能达到的组织和器官功能水平。它们还能够对功能组织和器官中活细胞的生物化学、遗传和代谢活动进行高分辨率、实时成像和体外分析。这项技术具有很大的潜力，以促进组织发育，器官生理学和疾病病因学的研究。在药物发现和开发的背景下，它应该是特别有价值的分子作用机制的研究，优先考虑的主要候选人，毒性测试和生物标志物鉴定。
An organ-on-a-chip is a microfluidic cell culture device created with microchip manufacturing methods that contains continuously perfused chambers inhabited by living cells arranged to simulate tissue-and organ-level physiology. By recapitulating the multicellular architectures, tissue-tissue interfaces, physicochemical microenvironments and vascular perfusion of the body, these devices produce levels of tissue and organ functionality not possible with conventional 2D or 3D culture systems. They also enable high-resolution, real-time imaging and in vitro analysis of biochemical, genetic and metabolic activities of living cells in a functional tissue and organ context. This technology has great potential to advance the study of tissue development, organ physiology and disease etiology. In the context of drug discovery and development, it should be especially valuable for the study of molecular mechanisms of action, prioritization of lead candidates, toxicity testing and biomarker identification.