Antibacterial activity and mechanism of Litsea cubeba essential oil against methicillin-resistant Staphylococcus aureus (MRSA)
Antibacterial activity and mechanism of Litsea cubeba essential oil against methicillin-resistant Staphylococcus aureus (MRSA)
复制标题
山苍子精油对耐甲氧西林金黄色葡萄球菌（MRSA）的抗菌活性及机制
DOI：
10.1016/j.indcrop.2018.12.078
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发表时间：
2019-04-01
影响因子：
5.9
通讯作者：
Lin, Lin
中科院分区：
文献类型：
--
作者：
Hu, Wei;Li, Changzhu;Lin, Lin
山苍子精油（LC-EO）是一种天然精油，具有良好的抑菌活性，在食品工业中有望逐步取代化学合成食品添加剂。然而，LC-EO的抗菌机理尚不确定。本研究以耐甲氧西林金黄色葡萄球菌（MRSA）为研究对象，探讨其抗菌机制。研究结果表明，LC-EO对MRSA细胞膜有破坏作用，导致细胞内生物大分子渗漏。LC-EO处理后MRSA的HMP途径及其关键酶葡萄糖-6-磷酸脱氢酶活性也受到抑制。最后，紫外吸收光谱结果表明，LC-EO的关键成分柠檬醛可以与DNA以嵌合体的形式发生反应。
Litsea cubeba essential oil (LC-EO), one of the natural essential oils, could gradually replace chemical synthesis food additive in food industry due to its good antimicrobial activity. However, the antibacterial mechanism of the LC-EO is uncertain. This study took methicillin-resistant staphylococcus aureus (MRSA) as the research object to exposit its antibacterial mechanism. Research findings indicated the destructive effect of LC-EO on MRSA cell membrane, leading to intracellular biological macromolecules leakage. What's more, the Hexose Monophophate Pathway (HMP) and its key enzyme (glucose-6-phosphate dehydrogenase) activity of MRSA were also inhibited after LC-EO treatment. Finally, the UV absorption spectroscopy result revealed that the key component of LC-EO (citral) could react with DNA in the form of chimera.