Clinical Significance of BTLA and HVEM Expression on Circulating CD4+ T and CD8+ T Cells in Chronic Hepatitis B Virus Infection.
Clinical Significance of BTLA and HVEM Expression on Circulating CD4+ T and CD8+ T Cells in Chronic Hepatitis B Virus Infection.
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DOI：
10.1089/vim.2021.0134
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发表时间：
2022-02
期刊：
影响因子：
2.2
通讯作者：
Hua-feng Song;Xiao-juan Chen;Pei-jun Tang;Ping Xu;Ziyi Huang;Xue-Feng Wang
中科院分区：
文献类型：
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作者：
Hua-feng Song;Xiao-juan Chen;Pei-jun Tang;Ping Xu;Ziyi Huang;Xue-Feng Wang
本研究通过检测慢性乙型病毒性肝炎(CHB)患者外周血中外周血中CD4+T细胞和CD8+T细胞表面B和T淋巴细胞衰减物(BTLA)和疱疹病毒进入介质(HVEM)的表达，探讨其与乙肝病毒临床指标的关系。与健康对照组比较，CHB患者外周血中CD4+T细胞和CD8+T细胞表面的BTLA和HVEM均显著上调(P<0.01)。有趣的是，在慢性乙型肝炎患者中，BTLA的表达与HIV-EM的表达呈正相关(CD_4+T细胞：r=0.5461，P<0.001；CD_8+T细胞：r=0.4206，P<0.01)。天冬氨酸氨基转移酶(T细胞：r=0.3136，P<0.05)、丙氨酸氨基转移酶(T细胞：r=0.3311，P<0.05)与BTLA表达呈正相关。CD8+T细胞：r=0.3325；CD8+T细胞：r=0.3476；然而，BTLA和HVEM表达的百分比与乙肝病毒载量之间没有明显的相关性。进一步的研究表明，在最佳的T细胞受体信号下，BTLA抑制信号可以显著抑制T细胞的增殖、激活和细胞因子的产生(p<；0.05)。因此，我们的研究结果表明，在T细胞耗竭过程中，T细胞表面BTLA和HVEM的表达增加可能具有一定的临床意义，并可能参与CHB的进展。
In this study, B and T lymphocyte attenuator (BTLA) and herpesvirus entry mediator (HVEM) expression on the surface of circulating CD4+ T and CD8+ T cells of patients with chronic hepatitis B (CHB) was investigated to explore their relationship with hepatitis B virus (HBV) clinical parameters. Both BTLA and HVEM were significantly upregulated on CD4+ T and CD8+ T cells of CHB patients compared with healthy controls (p < 0.01). Intriguingly, in CHB patients, the percentage of BTLA expression was positively correlated with that of HVEM (CD4+ T cells: r = 0.5461, p < 0.001 and CD8+ T cells: r = 0.4206, p < 0.01). Moreover, the percentage of BTLA expression was positively correlated with the levels of aspartate aminotransferase (AST) (CD4+ T cells: r = 0.3136, p < 0.05 and CD8+ T cells: r = 0.3159, p < 0.05) and alanine aminotransaminase (ALT) (CD4+ T cells: r = 0.3177, p < 0.05 and CD8+ T cells: r = 0.3311, p < 0.05). At the same time, the percentage of HVEM expression was also positively correlated with AST levels (CD4+ T cells: r = 0.3721, p < 0.05 and CD8+ T cells: r = 0.3325, p < 0.05) and ALT (CD4+ T cells: r = 0.3689, p < 0.05 and CD8+ T cells: r = 0.3476, p < 0.05). However, the percentage of BTLA and HVEM expression did not show significant relevance to HBV viral load. Further study demonstrated that BTLA inhibitory signaling could significantly inhibit T cell proliferation, activation, and cytokine production under optimal T cell receptor signaling (p < 0.05). Thereby, our findings indicate that the increased BTLA and HVEM expression on the surface of CD4+ and CD8+ T cells might represent a certain clinical significance and be involved in CHB progression during T cell exhaustion.