Detection of Anaplasma phagocytophilum from Wild Boars and Deer in Japan.
Detection of Anaplasma phagocytophilum from Wild Boars and Deer in Japan.
复制标题
DOI：
10.7883/yoken.64.333
复制
发表时间：
2011-07
影响因子：
2.2
通讯作者：
T. Masuzawa;Yoshiyuki Uchishima;T. Fukui;Y. Okamoto;M. Muto;N. Koizumi;A. Yamada
中科院分区：
文献类型：
--
作者：
T. Masuzawa;Yoshiyuki Uchishima;T. Fukui;Y. Okamoto;M. Muto;N. Koizumi;A. Yamada
本研究旨在确定日本野鹿和野猪中人粒细胞无形体病的病原体--嗜吞噬细胞无形体的流行率和地理分布。我们使用PCR靶向16 S rDNA，然后进行DNA测序来分析研究动物的血液样品。感染无形体属的野鹿，包括牛无浆体和中央无浆体在北海道至九州地区被检测到。A.嗜吞噬细胞A. bovis和A.鹿的感染率分别为15.6%、21.9%和37.5%，野猪的感染率分别为3.6%、17.9%和3.6%。然而，p44/msp 2基因特异于A. 16 SrDNA阳性标本中未检出嗜吞噬细胞菌。此外，还对A.与日本鹿的嗜吞噬细胞菌相似性为100%，与嗜吞噬细胞菌相似性为98.6%。在美国和欧洲检测到嗜吞噬细胞的硬蜱，在日本检测到卵形硬蜱和全沟硬蜱。这些结果表明，A.从日本鹿和野猪中检测到的嗜吞噬细胞相关序列与典型的嗜吞噬细胞相关序列不同。嗜吞噬细胞菌菌株。
This study was conducted to determine the prevalence and geographic distribution of Anaplasma phagocytophilum, the causative agent of human granulocytic anaplasmosis, in wild deer and boars in Japan. We analyzed the blood samples of the study animals using PCR-targeting the 16S rDNA followed by DNA sequencing. Wild deer infected with Anaplasma spp., including Anaplasma bovis and Anaplasma centrale were detected in the region from Hokkaido to Kyushu. The infection rates of A. phagocytophilum, A. bovis, and A. centrale in deer were 15.6, 21.9, and 37.5%, respectively, and the corresponding infection rates in wild boar were 3.6, 17.9, and 3.6%, respectively. However, p44/msp2 genes specific to A. phagocytophilum were not detected among the 16S rDNA-positive samples on PCR analysis. In addition, the 16S rDNA sequences of A. phagocytophilum were 100% similar to those detected previously in the deer in Japan and 98.6% similar to those of A. phagocytophilum detected in the United States and Europe, and from the tick, Ixodes ovatus and Ixodes persulcatus in Japan. These findings suggested that the A. phagocytophilum-related sequences detected from deer and wild boars in Japan were different from those of typical A. phagocytophilum strains.