Remote Effects of Transplanted Perivascular Adipose Tissue on Endothelial Function and Atherosclerosis.
Remote Effects of Transplanted Perivascular Adipose Tissue on Endothelial Function and Atherosclerosis.
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DOI：
10.1007/s10557-018-6821-y
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发表时间：
2018-10
影响因子：
3.4
通讯作者：
Weintraub NL
中科院分区：
文献类型：
--
作者：
Horimatsu T;Patel AS;Prasad R;Reid LE;Benson TW;Zarzour A;Ogbi M;Bruder do Nascimento T;Belin de Chantemele E;Stansfield BK;Lu XY;Kim HW;Weintraub NL
血管周围脂肪组织（PVAT）包裹着动脉外膜，在维持血管内环境稳定中起重要作用。PVAT在肥胖症中扩大，并且发炎的PVAT可局部促进内皮功能障碍和动脉粥样硬化。在这里，我们使用脂肪组织移植，测试了PVAT扩张也可以远程加重血管疾病的假设。从高脂饮食（HFD）喂养的野生型小鼠中分离50毫克腹主动脉PVAT，并移植到瘦型LDL受体敲除小鼠的腹主动脉上。皮下和内脏脂肪组织用作对照。HFD喂养10周后，测量体重、葡萄糖/胰岛素敏感性和脂质水平。在移植的脂肪组织中评估脂肪细胞因子基因表达，并收获胸主动脉以通过油红O染色来量化动脉粥样硬化病变，并通过钢丝肌造影来评估血管舒张。PVAT移植不影响体重、脂肪组成、脂质水平或葡萄糖/胰岛素敏感性。然而，与对照组相比，移植PVAT到腹主动脉增加胸主动脉粥样硬化。此外，PVAT移植到腹主动脉抑制胸主动脉内皮依赖性舒张。在移植的PVAT组织中，MCP-1和TNF-α的表达升高，而脂联素的表达降低，表明炎症增强是移植的PVAT的远端血管效应的潜在机制。这些数据表明，PVAT扩张和炎症在肥胖症可以远程诱导内皮功能障碍和增加动脉粥样硬化。确定潜在的机制可能会导致新的方法进行风险评估和治疗肥胖相关的血管疾病。
Perivascular adipose tissue (PVAT) surrounds the arterial adventitia and plays an important role in vascular homeostasis. PVAT expands in obesity, and inflamed PVAT can locally promote endothelial dysfunction and atherosclerosis. Here, using adipose tissue transplantation, we tested the hypothesis that expansion of PVAT can also remotely exacerbate vascular disease. Fifty milligrams of abdominal aortic PVAT was isolated from high-fat diet (HFD)-fed wild-type mice and transplanted onto the abdominal aorta of lean LDL receptor knockout mice. Subcutaneous and visceral adipose tissues were used as controls. After HFD feeding for 10 weeks, body weight, glucose/insulin sensitivity, and lipid levels were measured. Adipocytokine gene expression was assessed in the transplanted adipose tissues, and the thoracic aorta was harvested to quantify atherosclerotic lesions by Oil-Red O staining and to assess vasorelaxation by wire myography. PVAT transplantation did not influence body weight, fat composition, lipid levels, or glucose/insulin sensitivity. However, as compared with controls, transplantation of PVAT onto the abdominal aorta increased thoracic aortic atherosclerosis. Furthermore, PVAT transplantation onto the abdominal aorta inhibited endothelium-dependent relaxation in the thoracic aorta. MCP-1 and TNF-α expression was elevated, while adiponectin expression was reduced, in the transplanted PVAT tissue, suggesting augmented inflammation as a potential mechanism for the remote vascular effects of transplanted PVAT. These data suggest that PVAT expansion and inflammation in obesity can remotely induce endothelial dysfunction and augment atherosclerosis. Identifying the underlying mechanisms may lead to novel approaches for risk assessment and treatment of obesity-related vascular disease.
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影响因子：
7.8
作者：
Fleenor BS;Eng JS;Sindler AL;Pham BT;Kloor JD;Seals DR
通讯作者：
Seals DR
影响因子：
20.1
作者：
Chatterjee TK;Stoll LL;Denning GM;Harrelson A;Blomkalns AL;Idelman G;Rothenberg FG;Neltner B;Romig-Martin SA;Dickson EW;Rudich S;Weintraub NL
通讯作者：
Weintraub NL
影响因子：
4.8
作者：
Qiang S;Nakatsu Y;Seno Y;Fujishiro M;Sakoda H;Kushiyama A;Mori K;Matsunaga Y;Yamamotoya T;Kamata H;Asano T
通讯作者：
Asano T
血管周围脂肪组织来源的脂联素通过促进巨噬细胞自噬抑制项圈诱导的颈动脉粥样硬化。
影响因子：
3.7
作者：
Li C;Wang Z;Wang C;Ma Q;Zhao Y
通讯作者：
Zhao Y
DOI：
10.1161/atvbaha.114.303983
发表时间：
2014-08
期刊：
Arteriosclerosis, thrombosis, and vascular biology
影响因子：
--
作者：
Manka D;Chatterjee TK;Stoll LL;Basford JE;Konaniah ES;Srinivasan R;Bogdanov VY;Tang Y;Blomkalns AL;Hui DY;Weintraub NL
通讯作者：
Weintraub NL