Programmed cell death: I. Cytology of degeneration in the intersegmental muscles of the pernyi silkmoth
Programmed cell death: I. Cytology of degeneration in the intersegmental muscles of the pernyi silkmoth
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DOI：
10.1016/0022-1910(65)90099-5
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发表时间：
1965-01-01
期刊：
影响因子：
2.2
通讯作者：
WILLIAMS, CARROLL M.
中科院分区：
文献类型：
--
作者：
LOCKSHIN, RICHARD A.;WILLIAMS, CARROLL M.
在蚕pheraea pernyi中，腹部的腹部肌肉相互分解在旧p put虫的渗透后48小时内变性。通过光和电子显微镜研究了分解过程。在腹肌后的第5小时大约，第一个变性被视为肌原纤维侵蚀的散射区域，并伴随着线粒体的轻微收缩。到逐步分析的第10小时，人们可以检测出更大的变性和液泡区域，以及明显的肌质挤出。第10小时的电子微图显示了退化性髓磷脂图，线粒体缩小和线粒体附近的脂质沉积物。同时，据推测是溶酶体，肿胀和明显溶解。在附近，肌膜被侵蚀和破坏。到逐步分析后的第15小时，肌肉是脆弱的，非扣除的，体积减少了约三分之一；除了持续的Z波段外，肌原纤维和交叉裂缝变得混乱并消失，肌红丝的残留物附着在这些Z波段上。细胞核是甲状腺素，线粒体大大缩小和退化，溶酶体样的身体很少可见。肌肉在第48小时完全消失了。同时，神经肌肉连接处的突触前部分保持完整。肌肉的崩溃可以通过我们认为是看似可行的组织中溶酶体破裂所带来的细胞溶解来解释。此事件之后是肌原纤维的侵蚀和最终丧失，并通过细胞内细胞器的变性。 ||摘要作者：作者
In the silkworm, Antheraea pernyi, the inter segmental muscles of the abdomen degenerate within 48 hr after the ecdysis of the moth from the old pupal cuticle. The process of breakdown was studied by light and electron microscopy. At about the 5th hour after ecdysis, the first trace of degeneration is seen as scattered areas of erosion of the myofibrils, accompanied by a slight contraction of the mitochondria. By the 10th hour after ecdysis, one can detect larger areas of degeneration and vacuolization, and an apparent extrusion of sarcoplasm. Electron micro-graphs at the 10th hour show degenerative myelin figures, shrunken mitochondria, and deposits of lipid near the mitochondria. Meanwhile, small, darkly staining bodies, presumed to be lysosomes, undergo swelling and apparent dissolution; in their vicinity, the myofilaments are eroded and destroyed. By the 15th hour after ecdysis, the muscle is flaccid, non-contractile, and reduced in volume by about one-third; the myofibrils and cross-striations become disorganized and disappear, except for the persistent Z-bands, to which remnants of the myofilaments are attached. The nuclei are pycnotic, the mitochondria greatly shrunken and degenerate, and the lysosome-like bodies rarely visible. The muscle has completely disappeared by the 48th hour. Meanwhile, the pre-synaptic portion of the neuromuscular junctions remains intact. The breakdown of the muscles can be accounted for by cytolysis brought about by what we interpret to be the rupture of lysosomes in the apparently viable tissues. This event is followed by the erosion and eventual loss of the myofibrils and by the degeneration of the intracellular organelles. || ABSTRACT AUTHORS: Authors