Isolating "uncultivable" microorganisms in pure culture in a simulated natural environment
Isolating "uncultivable" microorganisms in pure culture in a simulated natural environment
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DOI：
10.1126/science.1070633
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发表时间：
2002-05-10
期刊：
影响因子：
56.9
通讯作者：
Epstein, SS
中科院分区：
文献类型：
--
作者：
Kaeberlein, T;Lewis, K;Epstein, SS
来自环境的大多数微生物(>；99%)抵制实验室培养。核糖体RNA分析表明，几乎在每个原核生物群中都发现了未培养的生物，而且有几个部门没有已知的可培养代表生物。我们设计了一个扩散室，允许以前未培养的微生物在模拟的自然环境中生长。在纯培养中分离出具有代表性的海洋生物的菌落。这些菌株不是单独在人工培养基上生长，而是在其他微生物存在的情况下形成菌落。这一观察结果可能有助于解释微生物不可培养的本质。
The majority (>99%) of microorganisms from the environment resist cultivation in the laboratory. Ribosomal RNA analysis suggests that uncultivated organisms are found in nearly every prokaryotic group, and several divisions have no known cultivable representatives. We designed a diffusion chamber that allowed the growth of previously uncultivated microorganisms in a simulated natural environment. Colonies of representative marine organisms were isolated in pure culture. These isolates did not grow on artificial media alone but formed colonies in the presence of other microorganisms. This observation may help explain the nature of microbial uncultivability.