Three developmental compartments involved in rib formation
Three developmental compartments involved in rib formation
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DOI：
10.1387/ijdb.041932ha
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发表时间：
2005-01-01
影响因子：
0.7
通讯作者：
Koseki, H
中科院分区：
文献类型：
--
作者：
Aoyama, H;Mizutani-Koseki, Y;Koseki, H
在2日龄鸡胚上，用铝箔将胸体节中胚层与神经管和脊索分离，术后7天，肋骨缺少近端部分。通过将脊索、神经管腹侧的一半或用Shh转化的QT 6细胞共移植到铝箔插入物的体节侧来拯救胚胎。因此，近端肋骨的发展取决于脊索和腹侧神经管，这可能是通过这些轴向组织分泌的Shh介导的效果。另一方面，当胸体节中胚层与表面外胚层由一片聚对苯二甲酸乙二醇酯膜分离时，肋骨的远端部分缺失，这表明远端肋骨发育依赖于表面外胚层。在这些胚胎中，Pax3的表达很弱，并且受到干扰，表明皮肌节发育异常。远侧肋的发育是由皮肌节还是外胚层介导的尚不清楚。以前的研究表明，胸肋的发育依赖于侧板中胚层。至于远侧肋，它被认为是由两部分组成。因此，肋骨是由三个发展舱室，在最近提出的分类体节衍生物的primaxial和abaxial。
When the thoracic somitic mesoderm was separated from the neural tube and the notochord with a piece of aluminum foil in two-day chick embryos, seven days after the operation ribs lacked their proximal part. The embryos were rescued by co-transplanting the notochord, the ventral half of neural tube, or QT6 cells transformed with Shh, on the somite side of the aluminum foil insert. Thus, proximal rib development depends on the notochord and the ventral neural tube, an effect which might be mediated through Shh secreted by these axial tissues. On the other hand, when the thoracic somitic mesoderm was separated from the surface ectoderm by a piece of polyethylene terephthalate film, the distal parts of the ribs were missing, suggesting that distal rib development depends on surface ectoderm. In these embryos, expression of Pax3 was weak and perturbed showing that the dermomyotome developed abnormally. It is not clear whether the development of distal rib is mediated by the dermomyotome, or the ectoderm. It has previously been shown that sternal rib development depends on lateral plate mesoderm. As to the distal rib, it is considered to be composed of two parts. Thus, the rib is composed of three developmental compartments, in agreement with a recently presented classification of somite derivatives as primaxial and abaxial.