Phytotoxin coronatine enhances heat tolerance via maintaining photosynthetic performance in wheat based on Electrophoresis and TOF-MS analysis.
Phytotoxin coronatine enhances heat tolerance via maintaining photosynthetic performance in wheat based on Electrophoresis and TOF-MS analysis.
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DOI：
10.1038/srep13870
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发表时间：
2015-09-08
影响因子：
4.6
通讯作者：
Duan L
中科院分区：
文献类型：
--
作者：
Zhou Y;Zhang M;Li J;Li Z;Tian X;Duan L
冠菌素 (COR) 是由丁香假单胞菌产生的植物毒素。它的结构与茉莉酸类似，在植物防御中发挥多种不同的作用。两者都具有 COI1 植物受体，因此冠菌素可以操纵植物激素信号传导以获取营养并抵消防御反应。除了激素系统外，冠菌素还影响植物氮代谢和叶绿体超微结构。在这项研究中，我们首先检查了典型的氮丢失表型，并使用聚丙烯酰胺凝胶方法在不同温度条件下的时程实验中展示了可溶性总蛋白模式。然后，我们采用三维凝胶电泳技术 (2-DE) 和 MALDI-TOF-MS 来隔离和鉴定敏感蛋白。我们总共发现了27个冠状素敏感蛋白，其中22个位于叶绿体中，其中6个直接参与光合作用。最后，我们测量了叶绿素和光合作用性能的水平，以揭示这些蛋白质的表型效应。综上所述，这些结果表明冠菌素通过调节氮代谢和叶绿体超微结构来增强耐热性，以维持光合性能并减少热胁迫下的产量损失。
Coronatine (COR) is a phytotoxin produced by Pseudomonas syringae. Its structure is similar to Jasmonates, which play a number of diverse roles in plant defense. Both have the COI1 plant receptor, so coronatine can manipulate plant hormone signaling to access nutrients and counteract defense responses. In addition to the hormone system, coronatine affects plant nitrogenous metabolism and chloroplast ultrastructure. In this study, we first examined a typical nitrogen-losing phenotype, and used the polyacrylamide gel approach to demonstrate soluble total protein patterns in a time-course experiment under different temperature conditions. We then employed dimensional gel electrophoresis technology (2-DE) and MALDI-TOF-MS to sequester and identify the sensitive proteins. We found a total of 27 coronatine sensitive proteins, 22 of which were located in the chloroplast and 6 of which were directly involved in photosynthesis. Finally, we measured levels of chlorophyll and photosynthetic performance to reveal the phenotypic effect of these proteins. Taken together, these results demonstrated that coronatine enhanced heat tolerance by regulating nitrogenous metabolism and chloroplast ultrastructure to maintain photosynthetic performance and reduce yield loss under heat stress.