Role of Drosophila gene dunc-115 in nervous system
Role of Drosophila gene dunc-115 in nervous system
复制标题
DOI：
10.1007/s10158-007-0047-1
复制
发表时间：
2007-06-01
影响因子：
--
通讯作者：
He, Qi
中科院分区：
文献类型：
--
作者：
Garcia, Melissa C.;Abbasi, Mohammed;He, Qi
轴突引导信号通过生长锥表面受体转导至内部，导致肌动蛋白动力学和肌动蛋白结合蛋白的变化，这对于确定肌动蛋白细胞骨架重组的结果至关重要。我们在此报告果蝇肌动蛋白结合蛋白 abLIM/Unc-115 同源物 Dunc-115 的特征及其在神经系统中的作用。三种 Dunc-115 同工型分别被鉴定为 Dunc-115L、M 和 S。 Dunc-115L 是 Unc-115 的典型同源物，具有四个 LIM 结构域和一个 villin 头结构域，而 Dunc-115M 和 S 是其他物种中没有对应物的新型亚型。我们的分子模型显示 Dunc-115L 可能与肌动蛋白结合。突变分析表明，Dunc-115 参与视觉和中枢神经系统的轴突投射。
Axonal guidance signals are transduced C through growth cone surface receptors to the interior leading to changes of actin dynamics and actin binding proteins, which are critical in determining the outcome of actin cytoskeleton reorganization. We report here the characterization of the Drosophila actin binding protein abLIM/Unc-115 homolog Dunc-115 and its role in the nervous system. Three Dunc-115 isoforms are identified as Dunc-115L, M and S, respectively. While Dunc-115L is a canonical homolog of Unc-115 with four LIM domains and one villin headpiece domain, Dunc-115M and S are novel isoforms without counterparts in other species. Our molecular modeling shows Dunc-115L is likely to bind to actin. Mutant analysis reveals that Dunc-115 is involved in axonal projection in both the visual and central nervous system.