Kinetic calibration using dominant pre-equilibrium desorption for on-site and in vivo sampling by solid-phase microextraction.
Kinetic calibration using dominant pre-equilibrium desorption for on-site and in vivo sampling by solid-phase microextraction.
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DOI：
10.1021/ac701871q
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发表时间：
2008-01
影响因子：
7.4
通讯作者：
S. Zhou;Wennan Zhao;J. Pawliszyn
中科院分区：
文献类型：
--
作者：
S. Zhou;Wennan Zhao;J. Pawliszyn
提出了一种新的固相微萃取主导预平衡解吸动力学校正方法。该校正是基于吸附和解吸之间的各向同性，这在水溶液和半固体基质中得到了理论和实验证明。这种方法允许使用解吸来补偿基质效应来校准吸收。此外，初步提出了浓度分布来验证吸附和解吸之间的各向同性，同时提供了一种线性方法来获得用于定量分析的时间常数。这种线性方法比以前使用的时间分布的非线性版本更方便、更稳健、更准确。此外，目标分析物被用作内标物；因此，不需要放射性或氚内标物。此外，主导预平衡解吸采用预平衡方法，样品制备时间更短，通常适合活体采样。该动力学校正方法已成功地应用于翡翠(Crassula Ovata)植物体内农药采样和流动系统中多环芳烃样品的制备。
A new kinetic calibration was developed using dominant pre-equilibrium desorption by solid-phase microextracton. The calibration was based on isotropism between absorption and desorption, which was proved theoretically and experimentally in an aqueous solution and semisolid matrix. This approach allows for the calibration of absorption using desorption to compensate for matrix effects. Moreover, concentration profiles are initially proposed to verify isotropism between the absorption and desorption, while providing a linear approach to obtain time constants for the purpose of quantitative analysis. This linear approach is more convenient, robust, and accurate than the nonlinear version with the previously used time profiles. Furthermore, the target analytes are used as the internal standards; thus, radioactive or deuterated internal standards are not necessary. In addition, dominant pre-equilibrium desorption utilizes the pre-equilibrium approach and offers a shorter sample preparation time, which is typically suitable for in vivo sampling. This kinetic calibration method was successfully applied to prepare samples of polycyclic aromatic hydrocarbons in a flow-through system and in vivo pesticide sampling in a jade plant (Crassula ovata).