Expression pattern of Wif1 and β-catenin during development of anorectum in fetal rats with anorectal malformations.
Expression pattern of Wif1 and β-catenin during development of anorectum in fetal rats with anorectal malformations.
复制标题
肛门直肠畸形胎鼠肛门直肠发育过程中Wifi和β-catenin的表达模式
作者：
Tang XB;Li H;Zhang J;Wang WL;Yuan ZW;Bai YZ
本研究旨在探讨Wnt抑制因子1 （Wnt inhibitory factor 1, Wif1）和β-catenin在正常和肛肠畸形（ano直肠畸形，ARM）胚胎肛肠发育过程中的表达规律，以及Wif1和β-catenin在ARM发病中的可能作用。在大鼠胚胎妊娠第10天用乙烯硫脲诱导ARM。通过剖宫产来获取胚胎。采用免疫组化染色、Western blot、实时RT-PCR等方法，对GD13 ~ GD16正常大鼠胚胎（n = 288）和ARM大鼠胚胎（n = 306）中Wif1、β-catenin蛋白及mRNA的表达模式进行检测。免疫组化染色显示，在正常胚胎中，从GD13到GD16， wip1在泄殖腔中持续表达。在GD13和GD14上，wip1免疫阳性细胞在泄殖腔中广泛表达。在GD15上，Wif1主要在极薄的肛膜上表达。在ARM胚胎中，从GD14到GD16，后肠和尿直肠隔上皮显示微弱的Wif1免疫染色。Western blot和real - time RT-PCR结果显示，与正常组相比，ARM组在GD14和GD15上的Wif1、β-catenin蛋白及mRNA表达水平显著降低（p < 0.05）。本研究发现，在肛肠形态发生过程中，ARM胚胎中Wif1和β-catenin的表达模式被破坏，这表明在肛肠分离进入原始直肠和泌尿生殖隔时，Wif1和β-catenin的下调可能与ARM的发育有关。
This study was performed to investigate the expression pattern of Wnt inhibitory factor 1 (Wif1) and β-catenin during anorectal development in normal and anorectal malformation (ARM) embryos and the possible role of Wif1 and β-catenin in the pathogenesis of ARM. ARM was induced with ethylenethiourea on the 10th gestational day in rat embryos. Cesarean deliveries were performed to harvest the embryos. The expression pattern of Wif1 and β-catenin protein and mRNA was evaluated in normal rat embryos (n = 288) and ARM rat embryos (n = 306) from GD13 to GD16 using immunohistochemical staining, Western blot, and real time RT-PCR. Immunohistochemical staining revealed that in normal embryos Wif1 was constantly expressed in the cloaca from GD13 to GD16. On GD13 and GD14, Wif1-immunopositive cells were extensively expressed in the cloaca. On GD15, the expression of Wif1 were mainly detected on the very thin anal membrane. In ARM embryos, the epithelium of the hindgut and urorectal septum demonstrated faint immunostaining for Wif1 from GD14 to GD16. Western blot and real time RT-PCR revealed that Wif1 and β-catenin protein and mRNA expression level was significantly decreased in the ARM groups compared with the normal group on GD14 and GD15 (p < 0.05). This study demonstrated that the expression pattern of Wif1 and β-catenin was disrupted in ARM embryos during anorectal morphogenesis, which demonstrated that downregulation of Wif1 and β-catenin at the time of cloacal separation into the primitive rectum and urogenital septum might related to the development of ARM.
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Wnt抑制因子1（WIF1）表达的失调导致内胚层内胚层的异常Wnt-β-catenin信号传导和细胞死亡以及肛门直肠畸形。
影响因子：
12.4
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影响因子：
2.4
作者：
Bai, YZ;Chen, H;Wang, WL
通讯作者：
Wang, WL
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1.7
作者：
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通讯作者：
Rintala, Risto J.
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2.8
作者：
Zhang, Tao;Bai, Yu Zuo;Wang, Wei Lin
通讯作者：
Wang, Wei Lin
影响因子：
4.6
作者：
Zhao, Tianyu;Gan, Qini;Zhou, Chengji J.
通讯作者：
Zhou, Chengji J.