Proteomic analysis of immature rat pups brain in response to hypoxia and ischemia challenge
Proteomic analysis of immature rat pups brain in response to hypoxia and ischemia challenge
复制标题
未成熟大鼠幼仔大脑响应缺氧和缺血挑战的蛋白质组学分析
DOI：
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发表时间：
2014
影响因子：
1.4
通讯作者：
Cui Hong
中科院分区：
文献类型：
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作者：
Yang Li-Jun;Ma Dong-Qing;Cui Hong
缺氧和缺血会显着影响围产期大脑发育，早产儿的情况更糟。然而，缺氧缺血发作后导致永久性脑损伤的机制细节尚未完全阐明。蛋白质组学可以深入了解潜在机制并有助于促进临床治疗。在这项研究中，使用液相色谱质谱联用无标记分析在缺氧缺血后 24 小时进行定量分析。与对照组相比，193 个蛋白质仅存在于缺氧缺血组中。此外，缺氧缺血组有34个蛋白上调2倍以上，14个蛋白下调2倍以上。 Gene Ontology数据库显示，大多数差异表达蛋白包括线粒体蛋白等。分子功能分析表明大多数蛋白质参与离子结合等。生物过程分析表明大多数蛋白质参与对有机物质等的反应。使用STRING 9.0软件分析来探索蛋白质之间存在的复杂相互作用。 Western blot验证了部分蛋白——微管相关蛋白2和微管相关蛋白tau的倍数变化。这项新颖的研究对幼鼠缺氧缺血性脑损伤的蛋白质组学研究进行了全面的筛选。
Hypoxia and ischemia significantly affects perinatal brain development, even worse in preterm infants. However, the details of the mechanism leading to permanent brain damage after hypoxia-ischemia attack have not been fully elucidated. Proteomics could provide insight into the potential mechanism and help to promote the clinical treatment. In this study, quantitative analysis was performed 24 hours after hypoxia-ischemia using liquidchromatography mass spectrometry coupled to label-free analysis. Compared to control, 193 proteins were present only in hypoxic-ischemic group. In addition, 34 proteins were more than 2 folds up-regulated and 14 proteins were more than 2 folds down-regulated in hypoxia-ischemia group. Gene Ontology database showed that the majority of differentially expressed proteins comprised mitochondrial proteins et al. Molecular function analysis revealed that the majority of proteins were involved in ion binding et al. Biological process analysis showed that the majority of proteins were involved in response to organic substance et al. STRING 9.0 software analysis were used to explore the complex interactions existed among the proteins. Western blot were used to verify the fold changes of some proteins-microtubule-associated protein 2 and microtubule-associated protein tau. This novel study performed a full-scale screening of the proteomics research in hypoxic-ischemic brain damage of immature rat.