Adjuvant and immuno-suppressive effect of six monophthalates in a subcutaneous injection model with BALB/c mice
Adjuvant and immuno-suppressive effect of six monophthalates in a subcutaneous injection model with BALB/c mice
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DOI：
10.1016/s0300-483x(01)00484-x
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发表时间：
2001-12-01
期刊：
影响因子：
4.5
通讯作者：
Nielsen, GD
中科院分区：
文献类型：
--
作者：
Larsen, ST;Hansen, JS;Nielsen, GD
在西欧和北美，过敏性气道疾病的患病率正在迅速增加。这种疾病流行率的增加可能与环境污染有关。本研究考察了一系列单邻苯二甲酸酯的佐剂和免疫抑制作用，这些单邻苯二甲酸酯被认为是常用邻苯二甲酸酯增塑剂的重要代谢产物。在筛选模型中研究了影响。在BALB/cJ小鼠颈部皮下注射卵清蛋白（OA）作为模型抗原，含或不含一种供试品，邻苯二甲酸单正丁酯（MnBP）、邻苯二甲酸单苄酯（MBO 2）、邻苯二甲酸单正辛酯（MnOP）、邻苯二甲酸单-2-乙基己酯（MEHP）、邻苯二甲酸单异壬酯（MiNP）或邻苯二甲酸单异癸酯（MiDP）。ELISA法检测血清中OA特异性IgE、IgG 1和IgG 2a水平。在MEHP（1000 μ g/ml，IgE和IgG 1）、MnOP（1000 μ g/ml，IgE和IgG 1）、MiNP（1000 μ g/ml，IgE和10 μ g/ml，IgG 1）和MiDP（100 μ g/ml，IgE和IgG 1）中观察到免疫抑制作用，定义为IgE或IgG 1抗体产生的统计学显著性降低。MEHP（10 μ g/ml，IgE）、MnOP（100 μ g/ml和10 μ g/ml，IgG 1）和MiNP（100 μ g/ml，IgE）发生佐剂效应，定义为IgE或IgG 1抗体水平的统计学显著性增加。用MBE 4和MnBP没有观察到统计学显著的免疫调节作用。(C)2001爱思唯尔科学爱尔兰有限公司保留所有权利。
The prevalence of allergic airway diseases is rapidly increasing in Western Europe and North America. This increase in disease prevalence may be associated with environmental pollutants. The present study investigated the adjuvant and immuno-suppressive effect of a series of monophthalates which are considered to be important metabolites of commonly used phthalate plasticizers. The effects were studied in a screening model. Ovalbumin (OA), used as the model antigen, was injected subcutaneously in the neck region of BALB/cJ mice with or without one of the test substances, mono-n-butyl phthalate (MnBP), monobenzyl phthalate (MBnP), mono-n-octyl phthalate (MnOP), mono-2-ethylhexyl phthalate (MEHP), mono-iso-nonyl phthalate (MiNP) or mono-iso-decyl phthalate (MiDP). The levels of OA-specific IgE, IgG1 and IgG2a in sera were measured by ELISA. Immuno-suppressive effect, defined as a statistically significant reduction in IgE or IgG1 antibody production, was observed with MEHP (1000 mug/ml, IgE and IgG1), MnOP (1000 mug/ml, IgE and IgG1), MiNP (1000 mug/ml, IgE and 10 mug/ml, IgG1) and MiDP (100 mug/ml, IgE and IgG1). Adjuvant effect, defined as a statistically significant increase in IgE or IgG1 antibody level, occurred with MEHP (10 mug/ml, IgE), MnOP (100 mug/ml, and 10 mug/ml, IgG1) and MiNP (100 mug/ml, IgE). No statistically significant immune modulating effect was seen with MBnP and MnBP. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.