The €100 lab: A 3D-printable open-source platform for fluorescence microscopy, optogenetics, and accurate temperature control during behaviour of zebrafish, Drosophila, and Caenorhabditis elegans.
The €100 lab: A 3D-printable open-source platform for fluorescence microscopy, optogenetics, and accurate temperature control during behaviour of zebrafish, Drosophila, and Caenorhabditis elegans.
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100欧元的实验室：一个可在斑马鱼，果蝇和秀丽隐杆线虫行为过程中，用于荧光显微镜，光遗传学和准确温度控制的3D打印机平台。
DOI：
10.1371/journal.pbio.2002702
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发表时间：
2017-07
期刊：
影响因子：
9.8
通讯作者：
Baden T
中科院分区：
文献类型：
--
作者：
Maia Chagas A;Prieto-Godino LL;Arrenberg AB;Baden T
小的、遗传上易驯化的物种，如斑马鱼幼体、果蝇或线虫，已经成为现代神经科学的关键模式生物。除了低维护成本和易于在实验室之间共享菌株外，一个关键的吸引力是可以监控行为领域中的单个或一组动物，同时使用光遗传或热遗传工具控制选定神经元的活动。然而，为这些类型的实验购买商业解决方案，包括适当的摄像系统和受控的行为竞技场，可能会很昂贵。在这里，我们提出了一个低成本、模块化的开源替代方案，称为‘FlyPI’。我们的设计基于3D打印主机、Raspberry PI计算机、高清晰度摄像头系统以及基于Arduino的光学和热控电路。根据配置的不同，FlyPI的组装价格可以低于100欧元，并且具有基于发光二极管(LED)的荧光显微镜和光遗传刺激的可选模块，以及用于热遗传学的基于Peltier的温度刺激器。带有所有模块的完整版本售价约为200欧元，如果用户准备四处选购的话，价格要低得多。FlyPI的所有功能都可以通过定制的图形用户界面进行控制。为了证明FlyPI的能力，我们介绍了它在一系列最先进的神经遗传学实验中的使用。此外，我们还展示了FlyPI作为一种医学诊断工具以及在几所非洲大学举办的神经遗传学课程中的教学辅助工具的实用性。总而言之，FlyPI的低成本和模块化特性以及完全开放的设计使其成为一种高度通用的工具，适用于包括教室、诊断中心和研究实验室在内的一系列应用。
Small, genetically tractable species such as larval zebrafish, Drosophila, or Caenorhabditis elegans have become key model organisms in modern neuroscience. In addition to their low maintenance costs and easy sharing of strains across labs, one key appeal is the possibility to monitor single or groups of animals in a behavioural arena while controlling the activity of select neurons using optogenetic or thermogenetic tools. However, the purchase of a commercial solution for these types of experiments, including an appropriate camera system as well as a controlled behavioural arena, can be costly. Here, we present a low-cost and modular open-source alternative called ‘FlyPi’. Our design is based on a 3D-printed mainframe, a Raspberry Pi computer, and high-definition camera system as well as Arduino-based optical and thermal control circuits. Depending on the configuration, FlyPi can be assembled for well under €100 and features optional modules for light-emitting diode (LED)-based fluorescence microscopy and optogenetic stimulation as well as a Peltier-based temperature stimulator for thermogenetics. The complete version with all modules costs approximately €200 or substantially less if the user is prepared to ‘shop around’. All functions of FlyPi can be controlled through a custom-written graphical user interface. To demonstrate FlyPi’s capabilities, we present its use in a series of state-of-the-art neurogenetics experiments. In addition, we demonstrate FlyPi’s utility as a medical diagnostic tool as well as a teaching aid at Neurogenetics courses held at several African universities. Taken together, the low cost and modular nature as well as fully open design of FlyPi make it a highly versatile tool in a range of applications, including the classroom, diagnostic centres, and research labs.