Effects of camptothecin and hydroxycamptothecin on insect cell lines Sf21 and IOZCAS-Spex-II.
Effects of camptothecin and hydroxycamptothecin on insect cell lines Sf21 and IOZCAS-Spex-II.
复制标题
喜树碱和羟基喜树碱对昆虫细胞系 Sf21 和 IOZCAS-Spex-II 的影响。
DOI：
10.1002/ps.2313
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发表时间：
2012-04
影响因子：
4.1
通讯作者：
Jiang, Hongyun
中科院分区：
文献类型：
--
作者：
Zhang, Lan;Zhang, Yanning;He, Weizhi;Ma, Dejun;Jiang, Hongyun
BACKGROUND
In recent years, concerns over the potential impact of synthetic pesticides on the environment have made the discovery and development of environmentally friendly pesticides a more pressing issue. Camptothecin (CPT) and its derivatives have attracted much attention owing to their unique mechanisms of action against insects. In this paper, two insect cell lines, Sf21 and IOZCAS-Spex-II, were used to conduct a preliminary investigation of the potential of CPT and hydroxycamptothecin (HCPT) as inducers of apoptosis in insect cell lines, and to illustrate the mechanism of action of CPT on insects at the cellular level.
RESULTS
The results showed that both CPT and HCPT demonstrate potent cytotoxic effects to the tested insect cell lines in a time- and dose-dependent manner. The DNA fragmentation, activation of caspases and cytochrome c release were observed in both IOZCAS-Spex-II and Sf21 treated with CPT and HCPT. There is no significant difference in cytotoxicity and caspase-3 activation (P < 0.05, except when treated for 2 h) between CPT and HCPT, although the caspase-3 activation was slightly stronger when treated with HCPT in both Sf21 and IOZCAS-Spex-II.
CONCLUSION
The results confirm the existence of the mitochondrial-dependent pathway of apoptosis induced by CPT and HCPT in Sf21 and IOZCAS-Spex-II cell lines. Further investigations are required to reveal the mitochondrial mechanisms and regulation of caspase activation during apoptosis. These studies will provide basic knowledge needed to understand the mechanisms of action of CPT and to develop CPT and its derivatives as insecticides.
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影响因子：
6.1
作者：
F. Zunino;G. Pratesi
通讯作者：
F. Zunino;G. Pratesi
影响因子：
10.4
作者：
BORKOVEC, AB
通讯作者：
BORKOVEC, AB
DOI：
10.1016/s0021-9258(17)38654-4
发表时间：
1985-11
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Yaw-Huei Hsiangl;Robert Hertzbergj;Sidney Hechtj;Leroy F. LiuS
通讯作者：
Yaw-Huei Hsiangl;Robert Hertzbergj;Sidney Hechtj;Leroy F. LiuS
影响因子：
5.6
作者：
Jesper Q. Svejstrup;K. Christiansen;Irina Gromova;A. H. Andersen;O. Westergaard
通讯作者：
Jesper Q. Svejstrup;K. Christiansen;Irina Gromova;A. H. Andersen;O. Westergaard
DOI：
10.1021/je900283v
发表时间：
2010-01-01
期刊：
JOURNAL OF CHEMICAL AND ENGINEERING DATA
影响因子：
--
作者：
Kunadharaju, Sasank;Savva, Michalakis
通讯作者：
Savva, Michalakis