The effects of graded levels of calorie restriction: IV. Non-linear change in behavioural phenotype of mice in response to short-term calorie restriction.
The effects of graded levels of calorie restriction: IV. Non-linear change in behavioural phenotype of mice in response to short-term calorie restriction.
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DOI：
10.1038/srep13198
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发表时间：
2015-08-25
影响因子：
4.6
通讯作者：
Speakman JR
中科院分区：
文献类型：
--
作者：
Lusseau D;Mitchell SE;Barros C;Derous D;Green C;Chen L;Han JD;Wang Y;Promislow DE;Douglas A;Speakman JR
当动物面临热量限制（CR）时，它们必须调整活动以应对能量摄入减少。如果CR是明显的，则变稳态可以将个体推入交替的生理状态，这可以在广泛的分类群中产生重要的健康益处。在这里，我们开发了一种新的方法来确定与不同水平的CR相关的行为表型的变化。我们将C57 BL/6雄性小鼠暴露于分级CR（从0到40%）三个月，并使用其运动和体温的隐马尔可夫模型来定义其行为表型。所有40%CR小鼠表现出行为表型的状态转变，只有一些暴露于30%CR。我们第一次表明，小鼠改变了它们的活动特征，而不是改变了它们的活动。这种新的表型分析方法提供了一种途径，以确定连接CR的健康跨度的机制。
Animals have to adjust their activities when faced with caloric restriction (CR) to deal with reduced energy intake. If CR is pronounced, allostasis can push individuals into alternate physiological states which can result in important health benefits across a wide range of taxa. Here we developed a new approach to determine the changes in behavioural phenotype associated with different levels of CR. We exposed C57BL/6 male mice to graded CR (from 0 to 40%) for three months and defined their behavioural phenotype using hidden Markov models of their movement and body temperature. All 40% CR mice exhibited a state-shift in behavioural phenotype and only some exposed to 30% CR did. We show for the first time that mice changed their activity characteristics rather than changed their activities. This new phenotyping approach provides an avenue to determine the mechanisms linking CR to healthspan.