POTENTIAL OF ISOLATED MOUSE INNER CELL MASSES TO FORM TROPHECTODERM DERIVATIVES INVIVO
POTENTIAL OF ISOLATED MOUSE INNER CELL MASSES TO FORM TROPHECTODERM DERIVATIVES INVIVO
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DOI：
10.1016/0012-1606(79)90022-8
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发表时间：
1979-01-01
影响因子：
2.7
通讯作者：
LIS, WT
中科院分区：
文献类型：
--
作者：
ROSSANT, J;LIS, WT
最近的体外实验表明：根据先前用于鉴定滋养外胚层衍生物的体外形态学实验，一些免疫手术分离的小鼠内细胞团（ICM）可能在初始囊胚形成后保留形成滋养外胚层的潜力。这些实验没有提供证据证明假定的滋养外胚层细胞能够在子宫内发挥作用。根据体内证据，至少从早期囊胚中分离出的 ICM 中的一些细胞确实保留了形成植入后滋养外胚层衍生物的潜力。早期 ICM 有时会导致 ICM/桑葚胚聚集嵌合体中的滋养层部分。当彼此聚集时，这些 ICM 能够植入子宫，这是滋养外胚层细胞独有的特性。一些聚集体重新构成了完整的蛋筒。从后来的囊胚中分离出的 ICM 很少在体内产生滋养层；形成滋养外胚层的能力明显在16-19细胞ICM阶段丧失。这些结果与早期发育中基因活动的变化模式相关。
Recent in vitro experiments have suggested that: Some immunosurgically isolated mouse inner cell masses (ICM) may retain the potential to form trophectoderm after initial blastocyst formation based on previous in vitro morphology experiments for identification of trophectoderm derivatives. These experiments provided no proof that the putative trophectoderm cells were capable of functioning in utero. At least some cells in ICM isolated from early blastocysts do retain the potential to form postimplantation trophectoderm derivatives based on in vivo evidence. Early ICM occasionally contributed to trophoblast fractions in ICM/morula aggregation chimeras. When aggregated with each other, these ICM were capable of implanting in the uterus, a property of trophectoderm cells alone. Some aggregates reconstituted complete egg cylinders. ICM isolated from later blastocysts rarely produced in vivo trophoblast; the ability to form trophectoderm apparently is lost around the 16-19 cell ICM stage. These results are discussed in relation to changing patterns of gene activity in early development.