CHRONIC EPILEPTIC FOCI IN NEOCORTEX - INVIVO AND INVITRO EFFECTS OF TETANUS TOXIN
CHRONIC EPILEPTIC FOCI IN NEOCORTEX - INVIVO AND INVITRO EFFECTS OF TETANUS TOXIN
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DOI：
10.1111/j.1460-9568.1991.tb00810.x
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发表时间：
1991-01-01
影响因子：
3.4
通讯作者：
GUTNICK, MJ
中科院分区：
文献类型：
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作者：
BRENER, K;AMITAI, Y;GUTNICK, MJ
将0.2-3.0 ng破伤风毒素注入大鼠顶叶新皮质，可导致该组织局部回路特性的永久性改变(7个月)。它导致了神经元活动的过度同步。这被认为是自发的阵发性场电位和/或诱发的全有或无群体爆发放电。从注射后16h到最长存活时间7个月，这种活动广泛地记录在注射后和对侧大脑半球的顶区和颞区。一些观察表明，超同步活动传播到对侧大脑半球的速度反映了毒素通过皮质轴突的运输，以及由此导致的突触抑制的阻断。然而，从已知的多肽在大脑中的半衰期来看，注射几个月后持续广泛分布的癫痫样放电不太可能是由于毒素的持续存在。因此，皮质内应用破伤风毒素提供了良好的慢性局灶性癫痫的实验模型，并提出了关于新皮质局部回路特性的长期调节的基本问题。
Injection of 0.2-3.0 ng of tetanus toxin into rat parietal neocortex resulted in permanent (> 7 months) changes in the local circuit properties of this tissue. It caused excessive synchronization of neuronal activity. This was seen as spontaneous paroxysmal field potentials and/or evoked all-or-none population burst discharges. Such activity was recorded widely over the parietal and temporal areas of both the injected and the contralateral hemispheres from as little as 16 h after injection up to the maximum survival time of 7 months. Several observations suggest that the speed with which the hypersynchronous activity spread to the opposite hemisphere reflects transport of the toxin through corticocortical axons and consequent blockade of synaptic inhibition. However, from what is known of the half life of the peptide in brain, it is unlikely that the persistent, widespread distribution of epileptiform discharge several months after injection was due to the continued presence of toxin. Thus, intracortical application of tetanus toxin provides good experimental model of chronic focal epilepsies, and raised fundamental questions regarding the long term regulation of local circuit properties in the neocortex.