ADF proteins are involved in the control of flowering and regulate F-actin organization, cell expansion, and organ growth in Arabidopsis
ADF proteins are involved in the control of flowering and regulate F-actin organization, cell expansion, and organ growth in Arabidopsis
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DOI：
10.1105/tpc.13.6.1333
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发表时间：
2001-06-01
期刊：
影响因子：
11.6
通讯作者：
Chua, NH
中科院分区：
文献类型：
--
作者：
Dong, CH;Xia, GX;Chua, NH
主要基于体外实验的结果，ADF（肌动蛋白解聚因子）蛋白被认为是肌动蛋白细胞骨架动态组织的关键调节剂。迄今为止报道的关于ADF蛋白体内功能的少数研究几乎完全使用单细胞系统进行，并且未能产生一致的结果。为了研究ADF在体内和多细胞器官发育过程中的功能，我们产生了表达编码ADF蛋白（AtADF 1）的cDNA的转基因拟南芥植物，该蛋白在强组成型活性启动子的控制下以有义或反义方向表达。选择AtADF蛋白表达水平显著改变的品系进行表型表征。AtADF 1的过表达导致不同类型细胞中粗肌动蛋白索的消失，导致不规则的细胞和组织形态发生，并降低细胞和器官的生长。相反，减少AtADF表达促进肌动蛋白电缆的形成，导致开花延迟，并刺激细胞扩张以及器官生长。这些结果与体外研究预测的ADF的分子功能一致，支持ADF蛋白在多细胞生物体发育过程中的全球作用，并证明这些蛋白是拟南芥中F-肌动蛋白组织，开花以及细胞和器官扩张的关键调节因子。
Based mostly on the results of in vitro experiments, ADF (actin-depolymerizing factor) proteins are thought to be key modulators of the dynamic organization of the actin cytoskeleton. The few studies concerned with the in vivo function of ADF proteins that have been reported to date were performed almost exclusively using single-cell systems and have failed to produce consistent results. To investigate ADF functions in vivo and during the development of multicellular organs, we generated transgenic Arabidopsis plants that express a cDNA encoding an ADF protein (AtADF1) in the sense or the antisense orientation under the control of a strong constitutively active promoter. Selected lines with significantly altered levels of AtADF protein expression were characterized phenotypically. Overexpression of AtADF1 resulted in the disappearance of thick actin cables in different cell types, caused irregular cellular and tissue morphogenesis, and reduced the growth of cells and organs. In contrast, reduced AtADF expression promoted the formation of actin cables, resulted in a delay in flowering, and stimulated cell expansion as well as organ growth. These results are consistent with the molecular functions of ADF as predicted by in vitro studies, support the global roles of ADF proteins during the development of a multicellular organism, and demonstrate that these proteins are key regulators of F-actin organization, flowering, and cell and organ expansion in Arabidopsis.