Genome-Wide Identification of the AP2/ERF Gene Family Involved in Active Constituent Biosynthesis in Salvia miltiorrhiza
Genome-Wide Identification of the AP2/ERF Gene Family Involved in Active Constituent Biosynthesis in Salvia miltiorrhiza
复制标题
参与丹参活性成分生物合成的 AP2/ERF 基因家族的全基因组鉴定
DOI：
10.3835/plantgenome2015.08.0077
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发表时间：
2016-07-01
期刊：
影响因子：
4.2
通讯作者：
Chen, S. L.
中科院分区：
文献类型：
--
作者：
Ji, A. J.;Luo, H. M.;Chen, S. L.
丹参酮和酚酸类化合物是传统药用植物丹参中的主要活性成分，然而转录因子对这些化合物生物合成的调控作用却鲜有报道。本文综述了S.本发明提供了丹参不同器官（根、茎、叶、花）和根组织（周皮、韧皮部、木质部）的发生、基因结构、保守基序和基因表达谱。共鉴定出170个AP 2/ERF基因，分为5个相对保守的亚家族，包括AP 2（25个基因）、DREB（61个基因）、乙烯应答因子（ERF; 79个基因）、RAV（4个基因）和Soloist（1个基因）。根据活性成分的分布和AP 2/ERF基因在不同器官和根组织中的表达模式，筛选出与活性成分生物合成相关的基因。通过实时荧光定量PCR（qRT-PCR）分析、共表达分析和启动子顺式调控元件预测，推测Sm 128和Sm 152基因参与丹参酮的生物合成，Sm 008和Sm 166基因参与酚酸的生物合成。与丹参酮生物合成相关的基因属于ERF-B3亚群。相反，预测调控酚酸生物合成的基因属于ERF-B1和ERF-B4亚组。这些结果为进一步研究AP 2/ERF基因的功能以促进S.丹参。
Tanshinones and phenolic acids are the major bioactive constituents in the traditional medicinal crop Salvia miltiorrhiza; however, transcription factors (TFs) are seldom investigated with regard to their regulation of the biosynthesis of these compounds. Here a complete overview of the APETALA2/ethylene-responsive factor (AP2/ERF) transcription factor family in S. miltiorrhiza is provided, including phylogeny, gene structure, conserved motifs, and gene expression profiles of different organs (root, stem, leaf, flower) and root tissues (periderm, phloem, xylem). In total, 170 AP2/ERF genes were identified and divided into five relatively conserved subfamilies, including AP2 (25 genes), DREB (61 genes), ethylene responsive factor (ERF; 79 genes), RAV (4 genes), and Soloist (1 gene). According to the distribution of bioactive constituents and the expression patterns of AP2/ERF genes in different organs and root tissues, the genes related to the biosynthesis of bioactive constituents were selected. On the basis of quantitative real-time polymerase chain reaction (qRT-PCR) analysis, coexpression analysis, and the prediction of cis-regulatory elements in the promoters, we propose that two genes (Sm128 and Sm152) regulate tanshinone biosynthesis and two genes (Sm008 and Sm166) participate in controlling phenolic acid biosynthesis. The genes related to tanshinone biosynthesis belong to the ERF-B3 subgroup. In contrast, the genes predicted to regulate phenolic acid biosynthesis belong to the ERF-B1 and ERF-B4 subgroups. These results provide a foundation for future functional characterization of AP2/ERF genes to enhance the biosynthesis of the bioactive compounds of S. miltiorrhiza.