Heme oxygenase 2: endothelial and neuronal localization and role in endothelium-dependent relaxation.
Heme oxygenase 2: endothelial and neuronal localization and role in endothelium-dependent relaxation.
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DOI：
10.1073/pnas.93.2.795
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发表时间：
1996-01
影响因子：
11.1
通讯作者：
R. Zakhary;S. Gaine;J. Dinerman;M. Ruat;N. Flavahan;S. Snyder
中科院分区：
文献类型：
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作者：
R. Zakhary;S. Gaine;J. Dinerman;M. Ruat;N. Flavahan;S. Snyder
合成一氧化碳 (CO) 的血红素加氧酶 2 (HO-2) 已通过免疫组织化学定位于血管内皮细胞和外膜神经。 HO-2 还定位于自主神经节中的神经元，包括岩神经节、颈上神经节和结状神经节，以及肠肌间神经丛中的神经节。酶研究表明，锡原卟啉-9 是 H2O 的选择性抑制剂，对 H2O 的选择性比内皮一氧化氮合酶 (NOS) 和可溶性鸟苷酸环化酶大约高 10 倍。锡原卟啉 9 对 H2O2 活性的抑制可逆转猪远端肺动脉内皮源性松弛的成分，而 NOS 抑制剂无法逆转这一成分。因此，CO 与 NO 一样，可能具有内皮源性舒张活性。 NOS 和 HO-2 在血管和自主神经系统中的定位和功能的相似性意味着这两种介质具有互补且可能协调的生理作用。
Heme oxygenase 2 (HO-2), which synthesizes carbon monoxide (CO), has been localized by immunohistochemistry to endothelial cells and adventitial nerves of blood vessels. HO-2 is also localized to neurons in autonomic ganglia, including the petrosal, superior cervical, and nodose ganglia, as well as ganglia in the myenteric plexus of the intestine. Enzyme studies demonstrated that tin protoporphyrin-9 is a selective inhibitor of HO with approximately 10-fold selectivity for HO over endothelial nitric oxide synthase (NOS) and soluble guanylyl cyclase. Inhibition of HO activity by tin protoporphyrin 9 reverses the component of endothelial-derived relaxation of porcine distal pulmonary arteries not reversed by an inhibitor of NOS. Thus, CO, like NO, may have endothelial-derived relaxing activity. The similarity of NOS and HO-2 localizations and functions in blood vessels and the autonomic nervous system implies complementary and possibly coordinated physiologic roles for these two mediators.