An in vivo method to prepare normal Schwann cells free of axons and myelin
An in vivo method to prepare normal Schwann cells free of axons and myelin
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一种体内制备不含轴突和髓磷脂的正常雪旺细胞的方法
DOI：
10.1016/0006-8993(79)90049-0
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发表时间：
1979
期刊：
影响因子：
2.9
通讯作者：
V. Zabrenetzky
中科院分区：
文献类型：
--
作者：
P. Spencer;H. J. Weinberg;V. Krygier;V. Zabrenetzky
从慢性失神经的周围神经中可以制备出可存活的未分化雪旺细胞。神经横断刺激远端残肢的一系列细胞事件，导致轴突和髓鞘的移除，以及雪旺细胞的增殖。封闭神经残端可防止神经再生，并使雪旺细胞子代驻留在纵向柱中。神经横断后5-12周，可从神经残端神经内膜组织中获取雪旺细胞。与从完整组织中制备的髓鞘细胞（其功能由相关轴突指定）不同，从失神经残肢中获得的雪旺细胞在功能上是幼稚的。因此，它们在分析髓鞘形成和有丝分裂的轴突调控方面是有用的。
A viable population of undifferentiated Schwann cells may be prepared from chronically denervated peripheral nerves. Nerve transection stimulates a sequence of cellular events in distal stumps leading to removal of axons and myelin, and proliferation of Schwann cells. Sealing the ends of nerve stumps prevents reinnervation and leaves daughter Schwann cells residing in longitudinal columns. Schwann cells may be harvested from the endoneurial tissue of the nerve stumps 5–12 weeks after nerve transection. Unlike myelinating cells prepared from intact tissue, where function has been specified by associated axons, Schwann cells obtained from denervated stumps are functionally naive. Their usefulness in analyzing axonal regulation of myelinogenesis and mitosis is therefore suggested.