Effect of Fiber Diameter and Alignment of Electrospun Polyurethane Meshes on Mesenchymal Progenitor Cells
Effect of Fiber Diameter and Alignment of Electrospun Polyurethane Meshes on Mesenchymal Progenitor Cells
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DOI：
10.1089/ten.tea.2008.0295
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发表时间：
2009-09-01
影响因子：
4.1
通讯作者：
Goldstein, Aaron S.
中科院分区：
文献类型：
--
作者：
Bashur, Chris A.;Shaffer, Robyn D.;Goldstein, Aaron S.
引导细胞排列和定向细胞外基质沉积的有效策略对于开发适用于修复韧带缺损的各向异性工程组织至关重要。静电纺丝是一种很有前途的方法，可以创建网格排列粘附细胞，但纤维网状结构对分化的影响还没有仔细检查。因此，本研究的目的是确定纤维直径和纤维排列程度对间充质祖细胞形态、增殖和韧带基因表达的影响。具体地，在设计为独立地改变平均纤维直径（从0.28至2.3 mm）和纤维排列程度的条件下，将聚（酯氨基甲酸酯）脲弹性体电纺到刚性载体上。骨髓基质细胞接种在支持的网状物上，粘附在所有表面并增殖。随着纤维直径和纤维排列程度的增加，细胞呈现更纺锤形的形态，并在对齐的网格上平行于纤维取向。韧带标志物胶原1 α 1、核心蛋白聚糖和腱调节蛋白的表达似乎对纤维直径敏感，并且在最小的纤维上最大。同时，转录因子scleraxis的表达似乎随着纤维排列的增加而减少。这些结果表明，当支架由排列的亚微米纤维组成时，静电纺丝支架上韧带样组织的形成得到增强。
Effective strategies to guide cell alignment and the deposition of an oriented extracellular matrix are critical for the development of anisotropic engineered tissues suitable for the repair of ligament defects. Electrospinning is a promising means to create meshes that can align adherent cells, but the effect of fiber mesh architecture on differentiation has not been examined closely. Therefore, the goal of this study was to determine the effect of fiber diameter and the degree of fiber alignment on mesenchymal progenitor cell morphology, proliferation, and ligament gene expression. Specifically, a poly(ester urethane) urea elastomer was electrospun onto rigid supports under conditions designed to independently vary the mean fiber diameter (from 0.28 to 2.3 mm) and the degree of fiber alignment. Bone marrow stromal cells-seeded onto supported meshes-adhered to and proliferated on all surfaces. Cells assumed a more spindle-shaped morphology with increasing fiber diameter and degree of fiber alignment, and oriented parallel to fibers on aligned meshes. Expression of the ligament markers collagen 1 alpha 1, decorin, and tenomodulin appeared to be sensitive to fiber diameter and greatest on the smallest fibers. Concurrently, expression of the transcription factor scleraxis appeared to decrease with increasing fiber alignment. These results suggest that the formation of a ligament-like tissue on electrospun scaffolds is enhanced when the scaffolds consist of aligned submicron fibers.