Time-lapse imaging of vitreoretinal angiogenesis originating from both quiescent and mature vessels in a novel ex vivo system.
Time-lapse imaging of vitreoretinal angiogenesis originating from both quiescent and mature vessels in a novel ex vivo system.
复制标题
新型离体系统中源自静止和成熟血管的玻璃体视网膜血管生成的延时成像。
DOI：
10.1167/iovs.06-0373
复制
发表时间：
2006
影响因子：
4.4
通讯作者：
N. Yoshimura
中科院分区：
文献类型：
--
作者：
T. Murakami;K. Suzuma;H. Takagi;M. Kita;H. Ohashi;Daisuke Watanabe;T. Ojima;M. Kurimoto;Tetsushi Kimura;A. Sakamoto;Noriharu Unoki;N. Yoshimura
目的
糖尿病视网膜病变（DR）是一种导致严重视力丧失的血管生成性疾病。然而，尚未描述增殖性糖尿病视网膜病变（PDR）中玻璃体视网膜新生血管形成的足够动物模型。本研究的目的是开发一种新颖的离体系统，用于评估源自静止和成熟血管的玻璃体视网膜血管生成过程，可以通过时间序列成像观察到。
方法
采用新方法将 7 至 8 周龄小鼠的视网膜培养 4 天，添加或不添加多种生长因子，并进行免疫组织化学分析。将 Tie2-GFP 小鼠的视网膜与血管内皮生长因子 (VEGF) 一起培养，并获得玻璃体视网膜血管生成的时序成像。
结果
血管萌芽由 VEGF 和胎盘生长因子诱导，但不由胰岛素样生长因子-1、碱性成纤维细胞生长因子或血管生成素-2 诱导。在有或没有 VEGF 的外植体中，血管周围壁细胞与内皮细胞分离，这是血管生成和 DR 进展过程中的重要步骤。此外，对视网膜新生血管形成事件的延时观察表明，从静止血管中出现血管芽的第一步是单细胞延伸。萌芽内皮细胞的前缘以连续的方式延伸和缩回。从新形成的血管中，出现了额外的血管芽，新血管彼此融合，导致血管分支。
结论
该系统的延时成像可视化静态和成熟血管玻璃体视网膜新生血管形成的动态过程。
PURPOSE
Diabetic retinopathy (DR) is an angiogenic disease that leads to severe visual loss. However, adequate animal models of vitreoretinal neovascularization in proliferative diabetic retinopathy (PDR) have not yet been described. The purpose of this study was to develop a novel ex vivo system for assessing vitreoretinal angiogenic processes that originate from both quiescent and mature vessels that could be observed with time-sequential imaging.
METHODS
The retinas of 7- to 8-week-old mice were cultured for 4 days, with or without several growth factors with novel procedures, and immunohistochemistry was performed. The retinas from Tie2-GFP mice were cultured with vascular endothelial growth factor (VEGF), and time-sequential imaging of vitreoretinal angiogenesis was acquired.
RESULTS
Vascular sprouts were induced by both VEGF and placenta growth factor, but not by insulin-like growth factor-1, basic fibroblast growth factor or angiopoietin-2. In explants with or without VEGF, perivascular mural cells were dissociated from endothelial cells, which is an important step during angiogenesis and in the progression of DR. Furthermore, use of time-lapse observations of retinal neovascularization events visualized that the first step in vascular sprout emergence from quiescent vessels was a single cell extension. The leading edges of a sprouting endothelial cell extended and retracted in a sequential manner. From newly formed vessels, additional vascular sprouts then emerged and new vessels fused to each other, resulting in vascular branching.
CONCLUSIONS
Time-lapse imaging of this system visualized the dynamic process in vitreoretinal neovascularization from quiescent and mature vessels.
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DOI：
10.1172/jci17808
发表时间：
2003-07
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
S. Shih;M. Ju;Nan Liu;Lois E. H. Smith
通讯作者：
S. Shih;M. Ju;Nan Liu;Lois E. H. Smith
影响因子：
4.4
作者：
Lois E. H. Smith;Eva Wesoloiuski;Angela McLellan;Sandra K. Kostyk;Robert D'Amato;Richard Sullivan
通讯作者：
Lois E. H. Smith;Eva Wesoloiuski;Angela McLellan;Sandra K. Kostyk;Robert D'Amato;Richard Sullivan
影响因子：
4.2
作者：
Frank, RN;Amin, RH;Abrams, GW
通讯作者：
Abrams, GW
DOI：
10.1073/pnas.92.3.905
发表时间：
1995-01-31
影响因子：
11.1
作者：
PIERCE, EA;AVERY, RL;SMITH, LEH
通讯作者：
SMITH, LEH
影响因子：
6
作者：
Ohno-Matsui, K;Hirose, A;Campochiaro, PA
通讯作者：
Campochiaro, PA